The impact of short-term machine perfusion on the risk of cancer recurrence after rat liver transplantation with donors after circulatory death

Oldani, Graziano; Peloso, Andréa; Slits, Florence; Gex, Quentin; Delaune, Vaihere; Orci, Lorenzo; Van De Looij, Yohan; Colin, Didier; Germain, Stéphane; De Vito, Claudio; Rubbia-Brandt, Laura; Lacotte, Stéphanie; Toso, Christian

doi:10.1371/journal.pone.0224890

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Title		The impact of short-term machine perfusion on the risk of cancer recurrence after rat liver transplantation with donors after circulatory death
Authors		Oldani, Graziano Peloso, Andréa Slits, Florence Gex, Quentin Delaune, Vaihere Orci, Lorenzo Van De Looij, Yohan Colin, Didier Germain, Stéphane De Vito, Claudio Rubbia-Brandt, Laura Lacotte, Stéphanie Toso, Christian show all authors [1 - 13]
Published in		PLOS ONE. 2019, vol. 14, no. 11, p. e0224890
Abstract		Hypothermic and normothermic ex vivo liver perfusions promote organ recovery after donation after circulatory death (DCD). We tested whether these perfusions can reduce the risk of hepatocellular carcinoma (HCC) recurrence in a 1h-DCD syngeneic transplantation model, using Fischer F344 rats. DCD grafts were machine perfused for 2h with hypothermic perfusion (HOPE) or normothermic perfusion (NORMO), and transplanted. After reperfusion, we injected HCC cells into the vena porta. On day 28 after transplantation, we assessed tumour volumes by MRI. Control rats included transplantations with Fresh and non-perfused DCD livers. We observed apoptotic-necrotic hepatocyte foci in all DCD grafts, which were more visible than in the Fresh liver grafts. Normothermic perfusion allowed a faster post-transplant recovery, with lower day 1 levels of transaminases compared with the other DCD. Overall, survival was similar in all four groups and all animals developed HCCs. Total tumor volume was lower in the Fresh liver recipients compared to the DCD and DCD+HOPE recipients. Volumes in DCD+NORMO recipients were not significantly different from those in the Fresh group. This experiment confirms that ischemia/reperfusion injury promotes HCC cell engraftment/growth after DCD liver transplantation. Using the present extreme 1h ischemia model, both hypothermic and normothermic perfusions were not effective in reducing this risk.
Identifiers		DOI: 10.1371/journal.pone.0224890 PMID: 31765399
Full text		Article (Published version) (2.2 MB) - Free access Supplemental data (856 Kb) - Free access Supplemental data (8.2 MB) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research group		Transplantation et hépatologie (905)
Project		FNS: PP00P3_139021
Citation (ISO format)		OLDANI, Graziano et al. The impact of short-term machine perfusion on the risk of cancer recurrence after rat liver transplantation with donors after circulatory death. In: PLOS ONE, 2019, vol. 14, n° 11, p. e0224890. doi: 10.1371/journal.pone.0224890 https://archive-ouverte.unige.ch/unige:127171