Scientific article
Open access

Simultaneous study of the recruitment of monocyte subpopulations under flow in vitro

Published inJournal of Visualized Experiments, no. 141
Publication date2018

The recruitment of monocytes from the blood to targeted peripheral tissues is critical to the inflammatory process during tissue injury, tumor development and autoimmune diseases. This is facilitated through a process of capture from free flow onto the luminal surface of activated endothelial cells, followed by their adhesion and transendothelial migration (transmigration) into the underlying affected tissue. However, the mechanisms that support the preferential and context-dependent recruitment of monocyte subpopulations are still not fully understood. Therefore, we have developed a method that allows the recruitment of different monocyte subpopulations to be simultaneously visualized and measured under flow. This method, based on time-lapse confocal imaging, allows for the unambiguous distinction between adherent and transmigrated monocytes. Here, we describe how this method can be used to simultaneously study the recruitment cascade of pro-angiogenic and non-angiogenic monocytes in vitro. Furthermore, this method can be extended to study the different steps of recruitment of up to three monocyte populations.

  • Cell Adhesion/physiology
  • Cell Movement/physiology
  • Cells
  • Cultured
  • Cohort Studies
  • Endothelium
  • Vascular/chemistry/cytology/physiology
  • Humans
  • Monocytes/chemistry/physiology
  • Time-Lapse Imaging/methods
  • Transendothelial and Transepithelial Migration/physiology
Citation (ISO format)
ROPRAZ, Patricia et al. Simultaneous study of the recruitment of monocyte subpopulations under flow in vitro. In: Journal of Visualized Experiments, 2018, n° 141. doi: 10.3791/58509
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Article (Published version)
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ISSN of the journal1940-087X

Technical informations

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