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Title

Mechanical ventilation alters the development of Staphylococcus aureus pneumonia in rabbit

Authors
Barbar, Saber-Davide
Pauchard, Laure-Anne
Bruyère, Rémi
Bruillard, Caroline
Hayez, Davy
Croisier, Delphine
Charles, Pierre-Emmanuel
Published in PLOS ONE. 2016, vol. 11, no. 7, p. e0158799
Abstract Ventilator-associated pneumonia (VAP) is common during mechanical ventilation (MV). Beside obvious deleterious effects on muco-ciliary clearance, MV could adversely shift the host immune response towards a pro-inflammatory pattern through toll-like receptor (TLRs) up-regulation. We tested this hypothesis in a rabbit model of Staphylococcus aureus VAP. Pneumonia was caused by airway challenge with S. aureus, in either spontaneously breathing (SB) or MV rabbits (n = 13 and 17, respectively). Pneumonia assessment regarding pulmonary and systemic bacterial burden, as well as inflammatory response was done 8 and 24 hours after S. aureus challenge. In addition, ex vivo stimulations of whole blood taken from SB or MV rabbits (n = 7 and 5, respectively) with TLR2 agonist or heat-killed S. aureus were performed. Data were expressed as mean±standard deviation. After 8 hours of infection, lung injury was more severe in MV animals (1.40±0.33 versus [vs] 2.40±0.55, p = 0.007), along with greater bacterial concentrations (6.13±0.63 vs. 4.96±1.31 colony forming units/gram, p = 0.002). Interleukin (IL)-8 and tumor necrosis factor (TNF)-αserum concentrations reached higher levels in MV animals (p = 0.010). Whole blood obtained from MV animals released larger amounts of cytokines if stimulated with TLR2 agonist or heat-killed S. aureus (e.g., TNF-α: 1656±166 vs. 1005±89; p = 0.014). Moreover, MV induced TLR2 overexpression in both lung and spleen tissue. MV hastened tissue injury, impaired lung bacterial clearance, and promoted a systemic inflammatory response, maybe through TLR2 overexpression.
Keywords AnimalsInterleukin-8/immunologyPneumoniaStaphylococcal/immunology/pathologyPneumoniaVentilator-Associated/immunology/microbiology/pathologyRabbitsRespirationArtificialStaphylococcus aureus/immunologyToll-Like Receptor 2/immunologyTumor Necrosis Factor-alpha/immunology
Identifiers
PMID: 27391952
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Research group Groupe Pugin Jérôme (Soins intensifs) (587)
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BARBAR, Saber-Davide et al. Mechanical ventilation alters the development of Staphylococcus aureus pneumonia in rabbit. In: PLOS ONE, 2016, vol. 11, n° 7, p. e0158799. doi: 10.1371/journal.pone.0158799 https://archive-ouverte.unige.ch/unige:126837

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Deposited on : 2019-11-25

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