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Book chapter
English

Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol

Published inStress Response: methods and protocols, Editors Walker J.M. & Keyse S.M., p. 321-345
PublisherNew Jersey : Humana Press
Collection
  • Methods in molecular biology; 99
Publication date2000
Abstract

An important component of the stress response, as well as many other biological systems, is the change in relative concentration of specific mRNAs following induction (1). Conventional techniques of differential screening may be sufficient for the detection and identification of some of these differentially expressed mRNAs, especially those of relatively high abundance. However, mRNAs displaying more subtle changes in their expression may be difficult to detect. This is particularly true for rare mRNAs showing relatively modest differences (10-fold or less) or for mRNAs expressed within only a subpopulation of cells in a sample.

Keywords
  • Polymerase chain reaction
  • Polymerase chain reaction amplification
  • Polymerase chain reaction reaction
  • Test population
  • Bromphenol blue
Citation (ISO format)
LAVERY, Daniel et al. Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol. In: Stress Response: methods and protocols. New Jersey : Humana Press, 2000. p. 321–345. (Methods in molecular biology) doi: 10.1385/1592590543
Main files (1)
Book chapter (Published version)
accessLevelRestricted
Identifiers
ISBN978-0-89603-611-6
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