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Scientific article
English

Chromatin Opening of DNA Satellites by Targeted Sequence-Specific Drugs

Published inMolecular Cell, vol. 6, no. 5, p. 999-1011
Publication date2000
Abstract

There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Drosophila melanogaster satellites. Dimeric oligopyrroles were shown to target the AT-rich satellites I, III, and SARs (scaffold associated regions). One polyamide (P31) specifically binds the GAGAA satellite V. Specificity of targeting was established by footprinting, epifluorescence of nuclei, and polytene chromosomes stained with fluorescent derivatives. These polyamides were shown to mediate satellite-specific chromatin opening of the chromatin fiber. Remarkably, certain polyamides induced defined gain or loss-of-function phenotypes when fed to Drosophila melanogaster.

Citation (ISO format)
JANSSEN, Samuel Théodoor, DURUSSEL, Thérèse, LAEMMLI, Ulrich Karl. Chromatin Opening of DNA Satellites by Targeted Sequence-Specific Drugs. In: Molecular Cell, 2000, vol. 6, n° 5, p. 999–1011. doi: 10.1016/S1097-2765(00)00099-X
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ISSN of the journal1097-2765
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