Book chapter (Published version) (381 Kb) - 
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Titles listDrug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction
Titles listDrug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction
Book Chapter
unige:126333 
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Drug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction |
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| Authors | ||
| Published in | L. Nathan Tumey. Antibody-Drug Conjugates Methods and Protocols. New York, NY: Springer US. 2020 | |
| Abstract | High-resolution native mass spectrometry (MS) provides accurate mass measurements (within 30 ppm) of intact ADCs and can also yield drug load distribution (DLD) and average drug to antibody ratio (DAR) in parallel with hydrophobic interaction chromatography (HIC). Native MS is furthermore unique in its ability to simultaneously detect covalent and noncovalent species in a mixture and for HIC peak identity assessment offline or online. As an orthogonal method described in this chapter, LC-MS following ADC reduction or IdeS (Fabricator) digestion and reduction can also be used to measure the DLD of light chain and Fd fragments for hinge native cysteine residues such as brentuximab vedotin. Both methods allow also the measurement of average DAR for both monomeric and multimeric species. In addition, the Fc fragments can be analyzed in the same run, providing a complete glycoprofile and the demonstration or absence of additional conjugation of this subdomain involved in FcRn and Fc-gammaR binding. | |
| Keywords | Brentuximab vedotin — DAR — DLD — Fabricator — HIC — IdeS — Mass spectrometry | |
| Identifiers | PMID: 31643057 ISBN: 978-1-4939-9928-6 | |
| Full text |
Book chapter (Published version) (381 Kb) - Limited access to UNIGE Other version: http://link.springer.com/10.1007/978-1-4939-9929-3_12 |
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| Structures | ||
| Research group | Sciences analytiques | |
| Citation (ISO format) | WAGNER-ROUSSET, Elsa et al. Drug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction. In: L. Nathan Tumey (Ed.). Antibody-Drug Conjugates Methods and Protocols. New York, NY : Springer US, 2020. doi: 10.1007/978-1-4939-9929-3_12 https://archive-ouverte.unige.ch/unige:126333 |
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