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Scientific article
English

Autologous Platelet-Rich Plasma (CuteCell PRP) Safely Boosts In Vitro Human Fibroblast Expansion

Published inTissue engineering. Part A, vol. 25, no. 21-22, p. 1550-1563
Publication date2019
Abstract

Autologous dermal fibroblast graft is an important therapy in skin defect repair, but in vitro skin cell culture is mandatory in most cases. However, cell expansion using xenogeneic/allogenic culture media presents some disadvantages, such as the risk of infection transmission. We demonstrated that an autologous culture system with the patient's own platelet-rich plasma is an efficient, cost-effective, and safe supplement for fibroblast culture. As it respects the good manufacturing practices and regulatory agencies standards, it should be considered as a potent alternative and substitute to xenogeneic or allogenic blood derivatives for the validation of future clinical protocols using in vitro cell expansion.

Keywords
  • Platelet-rich plasma
  • PRP
  • Fibroblasts
  • Cell proliferation
  • Cell migration
  • Wound healing
  • Cell culture
  • Cell therapy
Citation (ISO format)
BERNDT, Sarah et al. Autologous Platelet-Rich Plasma (CuteCell PRP) Safely Boosts In Vitro Human Fibroblast Expansion. In: Tissue engineering. Part A, 2019, vol. 25, n° 21-22, p. 1550–1563. doi: 10.1089/ten.TEA.2018.0335
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1937-3341
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Technical informations

Creation07/24/2019 11:40:00 AM
First validation07/24/2019 11:40:00 AM
Update time03/15/2023 6:22:34 PM
Status update03/15/2023 6:22:34 PM
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