UNIGE document Scientific Article
previous document  unige:126282  next document
add to browser collection
Title

Methods for detection of NOX-derived superoxide radical anion and hydrogen peroxide in cells

Authors
Published in Methods in Molecular Biology. 2019, vol. 1982, p. 233-241
Abstract NADPH oxidases (NOX) are transmembrane enzymes, which catalyze the formation of reactive oxygen species (ROS). In humans and most mammals, the NOX family comprises seven members, namely, NOX1-5 and the dual oxidases DUOX1 and 2. The primary product of most NOX isoforms is the superoxide radical anion O2ċ-, which is rapidly dismutated in hydrogen peroxide (H2O2), while NOX4 and DUOX mostly generate H2O2. ROS are multifunctional molecules in tissues, and NOX-derived ROS cellular functions are as diverse as microbial killing (NOX2), thyroid hormone synthesis (DUOX2), or otoconia formation in the inner ear (NOX3). NOX are potential pharmacological targets in numerous diseases such as diabetes, fibrosis, and brain ischemia, and NOX inhibitors are currently under development. Here we describe two cellular assays to detect extracellular O2ċ- and H2O2 in cells overexpressing specific NOX isoforms and their subunits.
Keywords Amplex redCellular assayHydrogen peroxideNADPH oxidasePeroxidaseSuperoxide radical anionWST-1
Identifiers
PMID: 31172475
Full text
Article (Published version) (285 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Radicaux libres et cellules souches embryonnaires (60)
Project
European Commission: NEURINOX
Citation
(ISO format)
AUGSBURGER, Fiona, FILIPPOVA, Aleksandra, JAQUET, Vincent. Methods for detection of NOX-derived superoxide radical anion and hydrogen peroxide in cells. In: Methods in Molecular Biology, 2019, vol. 1982, p. 233-241. doi: 10.1007/978-1-4939-9424-3_13 https://archive-ouverte.unige.ch/unige:126282

240 hits

3 downloads

Update

Deposited on : 2019-11-14

Export document
Format :
Citation style :