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ELAV mediates the repression of abd-A by transcriptional interference in the embryonic CNS of Drosophila

Defense date2019-09-09
Abstract

The iab-8 ncRNA of Drosophila melanogaster had previously been described as a repressor of the Hox gene abd-A in parasegment 13 of the embryonic central nervous system via both the production of a microRNA and a second, cis- acting mechanism, which was proposed to be transcriptional interference. Using genetic reporters to reproduce the interaction between the iab-8 ncRNA and abd-A, we confirmed this hypothesis. This mechanism is most likely dependent on the production of a CNS-specific isoform of the iab-8 ncRNA that invades the transcriptional unit of abd-A. The generation of this isoform relies on the activity of the RNA-binding protein ELAV that mediates alternative splicing of genes produced in neurons. Failure in the production of these transcripts in elav mutants causes derepression of abd-A in PS13 of the CNS. Based on these findings, we propose that ELAV mediates the repression of abd-A by transcriptional interference in the embryonic CNS of Drosophila.

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Keywords
  • NcRNA
  • Hox
  • Transcriptional interference
  • ELAV
  • Drosophila
  • Transcriptional readthrough
Citation (ISO format)
CASTRO ALVAREZ, Javier José. ELAV mediates the repression of abd-A by transcriptional interference in the embryonic CNS of Drosophila. 2019. doi: 10.13097/archive-ouverte/unige:124130
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Creation10/01/2019 12:13:00 PM
First validation10/01/2019 12:13:00 PM
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