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Title

Changes in Polypeptide Profiles of Two Pea Genotypes Inoculated with the Arbuscular Mycorrhizal Fungus Glomus mosseae

Authors
Samra, Assem
Dumas-Gaudot, Eliane
Gianinazzi-Pearson, Vivienne
Published in Azcon-Aguilar, Conception & Barea, José-Miguel. Mycorrhizas in integrated systems: from genes to plant development: proceedings of the 4. European symposium on mycorrhizas. Granada - 11 to 14 July 1994 - Luxembourg: Office for official publications of the european communities. 1996, p. 263-266
Abstract The symbiotic interaction between pea roots ('Pisum sativum' L.) and the endomycorrhizal fungus 'Glomus mosseae' should lead to specific gene expression of both symbionts. In order to detect symbiosis-related proteins (endomycorrhizins), we used two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) after phenolic extraction of total soluble proteins. Furthermore, to better characterize the molecular changes after fungal infection, two pea genotypes available in our laboratory were used: cv. Frisson (myc⁺) and an isogenic mycorrhiza-resistant mutant (myc⁻). The latter prevents intraradical fungal development. Several differences were observed in polypeptide patterns of silver-stained 2D gels between control and 'G. mosseae'-inoculated roots, after only 7 days of contact between both partners. Some polypeptides already present in control root extracts were altered with fungal colonization, being increased or decreased in intensity. Interestingly, new polypeptides were produced in 'G. mosseae'-infected roots from cv. Frisson whilst in the inoculated mycorrhiza-resistant mutant, different patterns were mainly characterized by disappearance of polypeptides compared to control roots.
Keywords Pisum sativumGlomus mosseaeMycorrhiza-resistant mutantSpecific polypeptides
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ISBN: 9789282756768
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Proceedings chapter (Published version) (2.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
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SAMRA, Assem et al. Changes in Polypeptide Profiles of Two Pea Genotypes Inoculated with the Arbuscular Mycorrhizal Fungus Glomus mosseae. In: Azcon-Aguilar, Conception & Barea, José-Miguel (Ed.). Mycorrhizas in integrated systems: from genes to plant development: proceedings of the 4. European symposium on mycorrhizas. Granada. Luxembourg : Office for official publications of the european communities, 1996. p. 263-266. https://archive-ouverte.unige.ch/unige:123628

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