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Comparison of Acid Soluble Protein Synthesis in Roots of Endomycorrhizal Wild Type Pisum sativum and Corresponding Isogenic Mutants

Published inJournal of Plant Physiology, vol. 141, no. 1, p. 2-6
Publication date1993
Abstract

The aim of this study was to compare profiles of acid soluble proteins in roots of Pisum sativum L. cv. Frisson and non-mycorrhizal mutants (myc−) when infected by vesicular arbuscular mycorrhiza funghi. Quantitative and qualitative modifications were detected by native or SDS PAGE in the protein composition of acidic extracts of endomycorrhizal roots of myc⁺, nod⁺ wild type P. sativum and of a myc⁺, nod-mutant, as compared to uninoculated control plants or a myc⁻, nod⁻ mutant, whether this was inoculated or not with Glomus mosseae. In native PAGE two proteins, already present in extracts of non-mycorrhizal pea roots, were enhanced in G. mosseae-infected myc⁺ plants (nod⁺ or nod⁻). One protein which appeared after inoculation with G. mosseae or G. fasciculatum in the roots of all pea plants, including the myc⁻, nod⁻ mutants, may be associated with appressorium formation. Four new proteins were detected in root extracts of G. mosseae-infected myc⁻ peas (nod⁺ or nod⁻), as compared to the myc⁻, nod⁻ mutant or uninoculated controls. One of these was probably of fungal origin, since it was also found in G. mosseae-infected tobacco and leek roots. These plants showed protein modifications different to those in endomycorrhizal peas, suggesting host-specific responses. SDS PAGE revealed the presence of two polypeptides of about 25 and 35 kDa in endomycorrhizal roots of myc⁺ peas. None of the endomycorrhiza-related proteins detected in roots corresponded to those extracted from spores of different fungi. This study provides new evidence that different molecular modifications occur during successful (myc⁺ pea, leek, tobacco) and unsuccessful (myc⁻ pea) endomycorrhiza infections, some of which are host-determined and others related to the expression of the fungal genome.

Keywords
  • Pisum sativum
  • Glomus species
  • Acaulospora laevis
  • Endomycorrhiza
  • myc⁻ nod⁻ mutants
  • Soluble proteins
Affiliation Not a UNIGE publication
Citation (ISO format)
SCHELLENBAUM, Luisa, GIANINAZZI, Silvio, GIANINAZZI-PEARSON, Vivienne. Comparison of Acid Soluble Protein Synthesis in Roots of Endomycorrhizal Wild Type <i>Pisum sativum</i> and Corresponding Isogenic Mutants. In: Journal of Plant Physiology, 1993, vol. 141, n° 1, p. 2–6. doi: 10.1016/S0176-1617(11)80843-6
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ISSN of the journal0176-1617
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