Detection of proteoforms using top-down mass spectrometry and diagnostic ions

Coelho Graca, Didia; Hartmer, Ralf; Jabs, Wolfgang; Scherl, Alexander; Clerici, Lorella; Samii, Kaveh; Tsybin, Yury O.; Hochstrasser, Denis; Lescuyer, Pierre

doi:10.1007/978-1-4939-9164-8_12

Book chapter

English

Detection of proteoforms using top-down mass spectrometry and diagnostic ions

ContributorsCoelho Graca, Didia; Hartmer, Ralf; Jabs, Wolfgang; Scherl, Alexander; Clerici, Lorella; Samii, Kaveh; Tsybin, Yury O.; Hochstrasser, Denis; Lescuyer, Pierre

Published inBrun, V. & Couté, Y. (Ed.), Proteomics for biomarker discovery : methods and protocols, p. 173-183

PublisherNew York, NY : Springer

Collection

Methods in molecular biology; 1959

Publication date2019

Abstract

Characterization of protein structure modifications is an important field in mass spectrometry (MS)-based proteomics. Here, we describe a process to quickly and reliably identify a mass change in a targeted protein sequence by top-down mass spectrometry (TD MS) using electron transfer dissociation (ETD). The step-by-step procedure describes how to develop a TD MS method for data acquisition as well as the data analysis process. The described TD MS workflow utilizes diagnostic ions to characterize an unknown sample in a few hours.

Keywords

ETD
Electron transfer dissociation
Hemoglobin
Hemoglobin disorders
Hemoglobin variant
Intact protein
Top-down

Affiliation entities

Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

Research groups

Citation (ISO format)

COELHO GRACA, Didia et al. Detection of proteoforms using top-down mass spectrometry and diagnostic ions. In: Proteomics for biomarker discovery : methods and protocols. Brun, V. & Couté, Y. (Ed.). New York, NY : Springer, 2019. p. 173–183. (Methods in molecular biology) doi: 10.1007/978-1-4939-9164-8_12

Book chapter (Published version)

Identifiers

PID : unige:122773
DOI : 10.1007/978-1-4939-9164-8_12
PMID : 30852823

Additional URL for this publicationhttp://link.springer.com/10.1007/978-1-4939-9164-8_12

ISBN978-1-4939-9163-1

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Creation03/09/2019 15:43:00

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Detection of proteoforms using top-down mass spectrometry and diagnostic ions

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