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Title

Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors

Authors
Roccio, Marta
Perny, Michael
Ealy, Megan
Widmer, Hans Ruedi
Heller, Stefan
Published in Nature Communications. 2018, vol. 9, no. 1, p. 4027
Abstract Sensory hair cells located in the organ of Corti are essential for cochlear mechanosensation. Their loss is irreversible in humans resulting in permanent hearing loss. The development of therapeutic interventions for hearing loss requires fundamental knowledge about similarities and potential differences between animal models and human development as well as the establishment of human cell based-assays. Here we analyze gene and protein expression of the developing human inner ear in a temporal window spanning from week 8 to 12 post conception, when cochlear hair cells become specified. Utilizing surface markers for the cochlear prosensory domain, namely EPCAM and CD271, we purify postmitotic hair cell progenitors that, when placed in culture in three-dimensional organoids, regain proliferative potential and eventually differentiate to hair cell-like cells in vitro. These results provide a foundation for comparative studies with otic cells generated from human pluripotent stem cells and for establishing novel platforms for drug validation.
Keywords Cell DifferentiationCell Separation/methodsCoculture TechniquesEpithelial Cell Adhesion Molecule/metabolismHumansNerve Tissue Proteins/metabolismOrgan of Corti/embryology/metabolismReceptorsNerve Growth Factor/metabolism
Identifiers
PMID: 30279445
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Other version: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168603/
Structures
Research group Groupe Pascal Senn (954)
Project FP7: OTOSTEM
Citation
(ISO format)
ROCCIO, Marta et al. Molecular characterization and prospective isolation of human fetal cochlear hair cell progenitors. In: Nature Communications, 2018, vol. 9, n° 1, p. 4027. https://archive-ouverte.unige.ch/unige:121806

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Deposited on : 2019-08-26

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