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Title

Dexamethasone-containing PLGA superparamagnetic microparticles as carriers for the local treatment of arthritis
Authors
Butoescu, Nicoleta Paula
Seemayer, Christian Alexander
Foti, Michelangelo
Jordan, Olivier
Doelker, Eric
Published in Biomaterials. 2009, vol. 30, no. 9, p. 1772-1780
Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) are attractive materials that have been widely used in medicine for diagnostic imaging and therapeutic applications. In our study, SPIONs and the corticosteroid dexamethasone acetate (DXM) are co-encapsulated into PLGA microparticles for the aim of locally treating inflammatory conditions such as arthritis. The magnetic properties conferred by the SPIONs could help to maintain the microparticles in the joint with an external magnet. The aim of this study was to investigate the interaction between magnetic microparticles and human synovial fibroblasts in terms of microparticle uptake (FACS, confocal and optical microscopy), internalization mechanism (Prussian Blue staining, TEM, immunofluorescence), cell toxicity (MTT) and tissue reaction after intra-articular injection (histology). The results show that the microparticles have an excellent biocompatibility with synoviocytes and that they are internalized through a phagocytic process, as demonstrated by fluorescence-activated cell sorting and morphological analyses of cells exposed to microparticles. Histological analysis showed that the prepared microparticles did not induce any inflammatory reaction in the joint. This type of carrier could represent a suitable magnetically retainable intra-articular drug delivery system for treating joint diseases such as arthritis or osteoarthritis.
Keywords Animals — Arthritis/*drug therapy — Cell Survival/drug effects — Cells, Cultured — Cytochalasin D/pharmacology — Dexamethasone/pharmacology/*therapeutic use — Drug Carriers/*chemistry — Endocytosis/drug effects — Ferrocyanides — Humans — Knee Joint/drug effects/pathology — Lactic Acid/*chemistry — *Magnetics — Mice — Nanoparticles/*chemistry/toxicity — Polyglycolic Acid/*chemistry — Synovial Membrane/drug effects/pathology/ultrastructure — Time Factors
Identifiers
PMID: 19135244
Full text
Article (Accepted version) (795 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271870&_user=9565874&_pii=S0142961208009885&_check=y&_ori...
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research group Obésité et syndrome métabolique (803)
Citation
(ISO format) 		BUTOESCU, Nicoleta Paula et al. Dexamethasone-containing PLGA superparamagnetic microparticles as carriers for the local treatment of arthritis. In: Biomaterials, 2009, vol. 30, n° 9, p. 1772-1780. doi: 10.1016/j.biomaterials.2008.12.017 https://archive-ouverte.unige.ch/unige:1216

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Deposited on : 2009-03-23

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