Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules

Lorenzo-Orts, Laura; Hohmann, Ulrich; Zhu, Jinsheng; Hothorn, Michael

doi:10.26508/lsa.201900385

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Title		Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules
Authors		Lorenzo-Orts, Laura Hohmann, Ulrich Zhu, Jinsheng Hothorn, Michael
Published in		Life Science Alliance. 2019, vol. 2, no. 3
Abstract		Inorganic polyphosphates (polyPs) are linear polymers of orthophosphate units linked by phosphoanhydride bonds. Here, we report that bacterial, archaeal, and eukaryotic conserved histidine α-helical (CHAD) domains are specific polyP-binding modules. Crystal structures reveal that CHAD domains are formed by two four-helix bundles, giving rise to a central pore surrounded by conserved basic surface patches. Different CHAD domains bind polyPs with dissociation constants ranging from the nano- to mid-micromolar range, but not nucleic acids. A CHAD-polyP complex structure reveals the phosphate polymer binding across the central pore and along the two basic patches. Mutational analysis of CHAD-polyP interface residues validates the complex structure. The presence of a CHAD domain in the polyPase ygiF enhances its enzymatic activity. The only known CHAD protein from the plant Ricinus communis localizes to the nucleus/nucleolus when expressed in Arabidopsis and tobacco, suggesting that plants may harbor polyPs in these compartments. We propose that CHAD domains may be used to engineer the properties of polyP-metabolizing enzymes and to specifically localize polyP stores in eukaryotic cells and tissues.
Identifiers		DOI: 10.26508/lsa.201900385 PMID: 31133615
Full text		Article (Published version) (3.9 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département des sciences végétales
Research group		Groupe Hothorn
Projects		Autre: International Research Scholar Award from the Howard Hughes Medical Institute European Commission: EUROPOLYPS
Citation (ISO format)		LORENZO-ORTS, Laura et al. Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. In: Life Science Alliance, 2019, vol. 2, n° 3. doi: 10.26508/lsa.201900385 https://archive-ouverte.unige.ch/unige:121406