Sample preparation development and matrix effects evaluation for multianalyte determination in urine

Marchi, Ivano Cedric; Rudaz, Serge; Veuthey, Jean-Luc

doi:10.1016/j.jpba.2008.11.040

Scientific article

English

Sample preparation development and matrix effects evaluation for multianalyte determination in urine

Contributors

Marchi, Ivano Cedric; Rudaz, Serge

; Veuthey, Jean-Luc

Published in

Journal of pharmaceutical and biomedical analysis, vol. 49, no. 2, p. 459-467

Publication date

2009

Abstract

The development of a generic analytical method remains difficult when a high number of compounds has to be simultaneously considered. This study proposes an innovative strategy for the development of a solid phase extraction (SPE) procedure before liquid chromatography-mass spectrometry analysis of 34 diuretics and beta-blockers in urine samples. These compounds have been selected since they are often encountered in anti-doping control. The principle is based on the selection of representative analytes during SPE protocol optimization, allowing a drastic reduction of generated data and development time. To select the representative compounds, all substances were classified based on their SPE behavior with a generic method and groups were formed with the help of a chemometric tool, namely hierarchical cluster analysis (HCA). One representative analyte per group was selected and used for subsequent SPE method development. Once the SPE method was developed, compounds were analyzed by LC-MS and matrix effects were evaluated to determine the influence of the matrix on the SPE process and MS signal alteration due to endogenous compounds. As a result, matrix effects evaluation must be performed on all analytes; representative compounds previously selected for SPE development were unable to predict matrix effects.

Keywords

Solid phase extraction
SPE
Fast SPE development
Representative compounds
Hierarchical cluster analysis
HCA

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

MARCHI, Ivano Cedric, RUDAZ, Serge, VEUTHEY, Jean-Luc. Sample preparation development and matrix effects evaluation for multianalyte determination in urine. In: Journal of pharmaceutical and biomedical analysis, 2009, vol. 49, n° 2, p. 459–467. doi: 10.1016/j.jpba.2008.11.040

Article (Accepted version)

Identifiers

PID : unige:1208
DOI : 10.1016/j.jpba.2008.11.040
PMID : 19157744

Journal ISSN

0731-7085

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Creation

23/02/2009 17:33:00

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23/02/2009 17:33:00

Update

14/03/2023 15:03:35

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14/03/2023 15:03:35

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18/03/2025 09:17:00

Sample preparation development and matrix effects evaluation for multianalyte determination in urine

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

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Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation