en
Scientific article
English

Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Published inThe journal of antimicrobial chemotherapy, vol. 62, no. 5, p. 948-950
Publication date2008
Abstract

OBJECTIVES: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland. METHODS: Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing. RESULTS: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes. CONCLUSIONS: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.

Keywords
  • Aeromonas/drug effects/isolation & purification
  • Anti-Bacterial Agents/pharmacology
  • DNA, Bacterial/genetics
  • Drug Resistance, Bacterial
  • Fresh Water/microbiology
  • Gene Order
  • Genes, Bacterial
  • Integrons
  • Interspersed Repetitive Sequences
  • Microbial Sensitivity Tests
  • Plasmids
  • Polymerase Chain Reaction
  • Quinolones/pharmacology
  • Sequence Analysis, DNA
  • Switzerland
Citation (ISO format)
PICÃO, Renata Cristina et al. Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake. In: The journal of antimicrobial chemotherapy, 2008, vol. 62, n° 5, p. 948–950. doi: 10.1093/jac/dkn341
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ISSN of the journal0305-7453
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