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Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Picão, Renata Cristina
Poirel, Laurent
Demarta, Antonella
Silva, Carla Sofia Ferreira
Petrini, Orlando
Nordmann, Patrice
Published in Journal of antimicrobial chemotherapy. 2008, vol. 62, no. 5, p. 948-50
Abstract OBJECTIVES: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland. METHODS: Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing. RESULTS: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes. CONCLUSIONS: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
Keywords Aeromonas/drug effects/isolation & purificationAnti-Bacterial Agents/pharmacologyDNA, Bacterial/geneticsDrug Resistance, BacterialFresh Water/microbiologyGene OrderGenes, BacterialIntegronsInterspersed Repetitive SequencesMicrobial Sensitivity TestsPlasmidsPolymerase Chain ReactionQuinolones/pharmacologySequence Analysis, DNASwitzerland
PMID: 18772162
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Other version: http://jac.oxfordjournals.org/content/62/5/948.long
Research group Acquisition et expression de facteurs de virulence chez Staphylococcus aureus (86)
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PICÃO, Renata Cristina et al. Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake. In: Journal of antimicrobial chemotherapy, 2008, vol. 62, n° 5, p. 948-50. doi: 10.1093/jac/dkn341 https://archive-ouverte.unige.ch/unige:12061

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Deposited on : 2010-10-11

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