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Title

Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Authors
Picão, Renata Cristina
Poirel, Laurent
Demarta, Antonella
Silva, Carla Sofia Ferreira
Petrini, Orlando
Nordmann, Patrice
Published in Journal of antimicrobial chemotherapy. 2008, vol. 62, no. 5, p. 948-50
Abstract OBJECTIVES: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland. METHODS: Isolates presenting MICs of nalidixic acid or ciprofloxacin > or = 1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing. RESULTS: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, bla(OXA-1), catB3 and arr-3 gene cassettes. CONCLUSIONS: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.
Keywords Aeromonas/drug effects/isolation & purificationAnti-Bacterial Agents/pharmacologyDNA, Bacterial/geneticsDrug Resistance, BacterialFresh Water/microbiologyGene OrderGenes, BacterialIntegronsInterspersed Repetitive SequencesMicrobial Sensitivity TestsPlasmidsPolymerase Chain ReactionQuinolones/pharmacologySequence Analysis, DNASwitzerland
Stable URL https://archive-ouverte.unige.ch/unige:12061
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Other version: http://jac.oxfordjournals.org/content/62/5/948.long
Identifiers
PMID: 18772162
Structures
Research group Acquisition et expression de facteurs de virulence chez Staphylococcus aureus (86)

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Deposited on : 2010-10-11

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