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Title

Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy

Authors
Amstad, Esther
Published in Langmuir. 2019, vol. 35, no. 26, p. 8748-8757
Abstract Horizontal black lipid membranes (BLMs) enable optical microscopy to be combined with the electrophysiological measurements for studying ion channels, peptide pores, and ionophores. However, a careful literature review reveals that simultaneous fluorescence and electrical recordings in horizontal BLMs have been rarely reported for an unclear reason, whereas many works employ bright-field microscopy instead of fluorescence microscopy or perform fluorescence imaging and electrical measurements one after another separately without truly exploiting the advantage of the combined setup. In this work, the major causes related to the simultaneous electrical and fluorescence recordings in horizontal BLMs are identified, and several solutions to counteract the issue are also proposed.
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Research groups Groupe Matile
Groupe Sugihara
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TSEMPEROULI, Maria et al. Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy. In: Langmuir, 2019, vol. 35, n° 26, p. 8748-8757. https://archive-ouverte.unige.ch/unige:120388

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Deposited on : 2019-07-05

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