en
Scientific article
English

Micro liquid chromatography coupled with evaporative light scattering detector at ambient and high temperature : optimization of the nebulization cell geometry

Published inJournal of chromatography, vol. 1192, p. 103-112
Publication date2008
Abstract

The recent developments in liquid chromatography (LC) are mainly dedicated to both system miniaturization (micro-, capillary-, and nano-LC) and analysis time decrease (fast-, and ultra-fast-LC). For the latter, several strategies can be used, and high temperature liquid chromatography (HTLC) seems very promising and easy to implement, especially in miniaturized system. In LC, the evaporative light scattering detector (ELSD) is considered an attractive alternative to conventional detector such as UV-vis due to its versatility and quasi-universality. Therefore, the compatibility of ELSD with mu-LC and mu-HTLC was investigated for several pharmaceutical compounds of interest. The nebulization process appeared to be the most critical parameter for performing the coupling and maintaining an efficient separation. Therefore, appropriate modifications in the nebulization cell geometry were brought to make ELSD fully compatible with mu-LC. The impact of optimized nebulization cell on chromatographic performance was evaluated in terms of efficiency and sensitivity. Finally, highly efficient, sensitive and fast separations of pharmaceutical drugs were performed with both techniques and the customized nebulization cell design.

Citation (ISO format)
GUILLARME, Davy et al. Micro liquid chromatography coupled with evaporative light scattering detector at ambient and high temperature : optimization of the nebulization cell geometry. In: Journal of chromatography, 2008, vol. 1192, p. 103–112. doi: 10.1016/j.chroma.2008.03.034
Identifiers
ISSN of the journal0021-9673
482views
0downloads

Technical informations

Creation10/29/2008 11:37:29 AM
First validation10/29/2008 11:37:29 AM
Update time03/14/2023 2:56:26 PM
Status update03/14/2023 2:56:26 PM
Last indexation01/16/2024 10:26:21 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack