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Proteomic profiling in an animal model of acute pancreatitis

Contributors

Fetaud, Vanessa; Frossard, Jean-Louis; Farina, Annarita; Pastor, Catherine; Buehler, Leo Hans; Dumonceau, Jean-Marc; Hadengue, Antoine; Hochstrasser, Denis; Lescuyer, Pierre

Published in

Proteomics, vol. 8, no. 17, p. 3621-3631

Publication date

2008

Abstract

Acute pancreatitis (AP) is an inflammatory disease of the pancreas, which evolves in approximately 20% of the patients to a severe illness associated with a high mortality rate. In this study, we performed a comparative proteomic analysis of pancreatic tissue extracts from rats with AP and healthy rodent controls in order to identify changes in protein expression related to the pathobiological processes of this disease. Pancreatic extracts from diseased and controls rats were analyzed by 2-DE and MS/MS. A total of 125 proteins were identified from both samples. Comparative analysis allowed the detection of 42 proteins or protein fragments differentially expressed between diseased and control pancreas, some of them being newly described in AP. Interestingly, these changes were representative of the main pathobiological pathways involved in this disease. We observed activation of digestive proteases and increased expression of various inflammatory markers, including several members of the alpha-macroglobulin family. We also detected changes related to oxidative and cell stress responses. Finally, we highlighted modifications of 14-3-3 proteins that could be related to apoptosis regulation. These results showed the interest of proteomic analysis to identify changes characterizing pancreatic tissue damage and, therefore, to highlight new potential biomarkers of AP.

Keywords

  • 14-3-3 Proteins/metabolism
  • Acute Disease
  • Animals
  • Antigens, Neoplasm/metabolism
  • Biological Markers/analysis
  • Caerulein
  • Disease Models, Animal
  • Electrophoresis, Gel, Two-Dimensional
  • Lectins, C-Type/metabolism
  • Lithostathine/metabolism
  • Male
  • Oxidative Stress/physiology
  • Pancreas/chemistry
  • Pancreatitis/chemically induced/metabolism
  • Proteomics
  • Rats
  • Rats, Sprague-Dawley
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tandem Mass Spectrometry
  • Tumor Markers, Biological/metabolism

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

FETAUD, Vanessa et al. Proteomic profiling in an animal model of acute pancreatitis. In: Proteomics, 2008, vol. 8, n° 17, p. 3621–3631. doi: 10.1002/pmic.200800066

Main files (1)

Article (Accepted version)

Identifiers

Journal ISSN

1615-9853
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Technical informations

Creation

19/03/2009 14:52:00

First validation

19/03/2009 14:52:00

Update

26/03/2026 10:43:13

Status update

26/03/2026 10:43:13

Last indexation

26/03/2026 10:48:31
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