UNIGE document Scientific Article
previous document  unige:1195  next document
add to browser collection
Title

Proteomic profiling in an animal model of acute pancreatitis

Authors
Fetaud, Vanessa
show hidden authors show all authors [1 - 9]
Published in Proteomics. 2008, vol. 8, no. 17, p. 3621-31
Abstract Acute pancreatitis (AP) is an inflammatory disease of the pancreas, which evolves in approximately 20% of the patients to a severe illness associated with a high mortality rate. In this study, we performed a comparative proteomic analysis of pancreatic tissue extracts from rats with AP and healthy rodent controls in order to identify changes in protein expression related to the pathobiological processes of this disease. Pancreatic extracts from diseased and controls rats were analyzed by 2-DE and MS/MS. A total of 125 proteins were identified from both samples. Comparative analysis allowed the detection of 42 proteins or protein fragments differentially expressed between diseased and control pancreas, some of them being newly described in AP. Interestingly, these changes were representative of the main pathobiological pathways involved in this disease. We observed activation of digestive proteases and increased expression of various inflammatory markers, including several members of the alpha-macroglobulin family. We also detected changes related to oxidative and cell stress responses. Finally, we highlighted modifications of 14-3-3 proteins that could be related to apoptosis regulation. These results showed the interest of proteomic analysis to identify changes characterizing pancreatic tissue damage and, therefore, to highlight new potential biomarkers of AP.
Keywords 14-3-3 Proteins/metabolismAcute DiseaseAnimalsAntigens, Neoplasm/metabolismBiological Markers/analysisCaeruleinDisease Models, AnimalElectrophoresis, Gel, Two-DimensionalLectins, C-Type/metabolismLithostathine/metabolismMaleOxidative Stress/physiologyPancreas/chemistryPancreatitis/chemically induced/metabolismProteomicsRatsRats, Sprague-DawleySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTandem Mass SpectrometryTumor Markers, Biological/metabolism
Stable URL https://archive-ouverte.unige.ch/unige:1195
Full text
Identifiers
PMID: 18686302
Structures
Research groups La protéomique au service de la clinique (270)
Cultures cellulaires et transplantations (519)
Physiopathologie de la pancréatite aiguë (601)
Pharmacologie et imagerie du foie (664)

198 hits

14 downloads

Update

Deposited on : 2009-03-20

Export document
Format :
Citation style :