• Home
  1. Home
  2. Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis
Scientific article
English

Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis

Contributors

Baudino, Lucie Clementine; Yoshinobu, Kumiko; Morito, Naoki; Kikuchi, Shuichi; Fossati-Jimack, Liliane; Morley, Bernard J.; Vyse, Timothy J.; Hirose, Sachiko; Jørgensen, Trine N.; Tucker, Rebecca M.; Roark, Christina L.; Kotzin, Brian L.; Evans, Leonard H.; Izui, Shozo

Published in

The Journal of immunology, vol. 181, no. 4, p. 2846-2854

Publication date

2008

Abstract

The endogenous retroviral envelope glycoprotein, gp70, implicated in murine lupus nephritis is secreted by hepatocytes as an acute phase protein, and it has been thought to be a product of an endogenous xenotropic virus, NZB-X1. However, since endogenous polytropic (PT) and modified polytropic (mPT) viruses encode gp70s that are closely related to xenotropic gp70, these viruses can be additional sources of serum gp70. To better understand the genetic basis of the expression of serum gp70, we analyzed the abundance of xenotropic, PT, or mPT gp70 RNAs in livers and the genomic composition of corresponding proviruses in various strains of mice, including two different Sgp (serum gp70 production) congenic mice. Our results demonstrated that the expression of different viral gp70 RNAs was remarkably heterogeneous among various mouse strains and that the level of serum gp70 production was regulated by multiple structural and regulatory genes. Additionally, a significant contribution of PT and mPT gp70s to serum gp70 was revealed by the detection of PT and mPT, but not xenotropic transcripts in 129 mice, and by a closer correlation of serum levels of gp70 with the abundance of PT and mPT gp70 RNAs than with that of xenotropic gp70 RNA in Sgp3 congenic mice. Furthermore, the injection of lipopolysaccharides selectively up-regulated the expression of xenotropic and mPT gp70 RNAs, but not PT gp70 RNA. Our data indicate that the genetic origin of serum gp70 is more heterogeneous than previously thought, and that distinct retroviral gp70s are differentially regulated in physiological vs inflammatory conditions.

Keywords

  • Animals
  • Autoantigens/biosynthesis/genetics/immunology
  • Endogenous Retroviruses/genetics/immunology
  • Glycoproteins/biosynthesis/blood/genetics
  • Inflammation Mediators/blood/physiology
  • Lupus Nephritis/blood/genetics/pathology
  • Mice
  • Mice, Congenic
  • Mice, Inbred C57BL
  • Mice, Inbred MRL lpr
  • Mice, Inbred NZB
  • Molecular Chaperones/biosynthesis/genetics

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

BAUDINO, Lucie Clementine et al. Dissection of genetic mechanisms governing the expression of serum retroviral gp70 implicated in murine lupus nephritis. In: The Journal of immunology, 2008, vol. 181, n° 4, p. 2846–2854. doi: 10.4049/jimmunol.181.4.2846

Main files (1)

Article (Accepted version)

Identifiers

Additional URL for this publication

http://www.jimmunol.org/cgi/content/full/181/4/2846

Journal ISSN

0022-1767
646views
0downloads

Technical informations

Creation

19/03/2009 14:23:00

First validation

19/03/2009 14:23:00

Update

14/03/2023 15:03:30

Status update

14/03/2023 15:03:30

Last indexation

29/10/2024 11:22:59
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated