Scientific Article
previous document  unige:118138  next document
add to browser collection

Robust postmortem survival of murine vestibular and cochlear stem cells

Oshima, Kazuo
Teo, Dawn
Grimm, Christian
Heller, Stefan
Published in JARO - Journal of the Association for Research in Otolaryngology. 2007, vol. 8, no. 2, p. 194-204
Abstract Potential treatment strategies of neurodegenerative and other diseases with stem cells derived from nonembryonic tissues are much less subjected to ethical criticism than embryonic stem cell-based approaches. Here we report the isolation of inner ear stem cells, which may be useful in cell replacement therapies for hearing loss, after protracted postmortem intervals. We found that neonatal murine inner ear tissues, including vestibular and cochlear sensory epithelia, display remarkably robust cellular survival, even 10 days postmortem. Similarly, isolation of sphere-forming stem cells was possible up to 10 days postmortem. We detected no difference in the proliferation and differentiation potential between stem cells isolated directly after death and up to 5 days postmortem. At longer postmortem intervals, we observed that the potency of sphere-derived cells to spontaneously differentiate into mature cell types diminishes prior to the cells losing their potential for self-renewal. Three-week-old mice also displayed sphere-forming stem cells in all inner ear tissues investigated up to 5 days postmortem. In summary, our results demonstrate that postmortem murine inner ear tissue is suited for isolation of stem cells.
Keywords AnimalsCell SurvivalCochlea/cytologyDeathMiceStem Cells/physiologyVestibuleLabyrinth/cytology
PMID: 17334849
Full text
(ISO format)
SENN, Pascal et al. Robust postmortem survival of murine vestibular and cochlear stem cells. In: JARO - Journal of the Association for Research in Otolaryngology, 2007, vol. 8, n° 2, p. 194-204.

42 hits

0 download


Deposited on : 2019-05-23

Export document
Format :
Citation style :