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Title

Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers

Authors
Hobby, K.R.
Major, H.J.
Published in Journal of Chromatography. A. 2001, vol. 926, p. 29-41
Abstract LC-MS-MS is becoming a very important tool for the on-line identification of natural products in crude plant extracts. For an efficient use of this technique in the dereplication of natural products, a careful study of the parameters used to generate informative MS-MS spectra is needed. In this paper, the collision-induced dissociation (CED) MS-MS spectra of ubiquitous C-glycosidic flavonoids have been systematically studied using hybrid quadrupole time-of-flight and ion-trap (IT) mass analysers under various CID energy conditions. Efficient differentiation of flavonoid C-glycoside isomers was possible, based on the comparison of CID-MS-MS spectra of particular C-glycoside unit fragments. Striking differences between 6-C and 8-C flavonoid glycosides were especially observed in the product ion spectra of their X-0,2(+) fragments ([M+H-120](+)). Some guidelines for the on-line characterisation of C-glycosidic flavonoids by LC-MS-MS or LC-multiple-stage MS are given.
Keywords mass spectrometrycollision-induced dissociationflavonoidsglycosidesfast-atom-bombardmentperformance liquid-chromatographyquantitative-analysisplant constituentsidentificationarrayflavanonesaglyconesmsuv.
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WARIDEL, Patrice et al. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. In: Journal of Chromatography. A, 2001, vol. 926, p. 29-41. https://archive-ouverte.unige.ch/unige:11813

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Deposited on : 2010-09-10

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