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Scientific article
English

Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers

Published inJournal of chromatography, vol. 926, p. 29-41
Publication date2001
Abstract

LC-MS-MS is becoming a very important tool for the on-line identification of natural products in crude plant extracts. For an efficient use of this technique in the dereplication of natural products, a careful study of the parameters used to generate informative MS-MS spectra is needed. In this paper, the collision-induced dissociation (CED) MS-MS spectra of ubiquitous C-glycosidic flavonoids have been systematically studied using hybrid quadrupole time-of-flight and ion-trap (IT) mass analysers under various CID energy conditions. Efficient differentiation of flavonoid C-glycoside isomers was possible, based on the comparison of CID-MS-MS spectra of particular C-glycoside unit fragments. Striking differences between 6-C and 8-C flavonoid glycosides were especially observed in the product ion spectra of their X-0,2(+) fragments ([M+H-120](+)). Some guidelines for the on-line characterisation of C-glycosidic flavonoids by LC-MS-MS or LC-multiple-stage MS are given.

Keywords
  • Mass spectrometry
  • Collision-induced dissociation
  • Flavonoids
  • Glycosides
  • Fast-atom-bombardment
  • Performance liquid-chromatography
  • Quantitative-analysis
  • Plant constituents
  • Identification
  • Array
  • Flavanones
  • Aglycones
  • MS
  • UV
Affiliation Not a UNIGE publication
Citation (ISO format)
WARIDEL, Patrice et al. Evaluation of quadrupole time-of-flight tandem mass spectrometry and ion-trap multiple-stage mass spectrometry for the differentiation of C-glycosidic flavonoid isomers. In: Journal of chromatography, 2001, vol. 926, p. 29–41.
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
  • PID : unige:11813
ISSN of the journal0021-9673
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