Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

Islam, N.; Parveen, S.A.; Nakazawa, N.; Marston, Andrew; Hostettmann, Kurt

doi:10.1080/13880200390502621

Scientific article

English

Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

ContributorsIslam, N.; Parveen, S.A.; Nakazawa, N.; Marston, Andrew; Hostettmann, Kurt

Published inPharmaceutical biology, vol. 41, no. 8, p. 637-640

Publication date2003

Abstract

A simple bioautographic agar spray assay has been developed using the plant pathogenic fungus Valsa ceratosperma as an indicator organism for the detection and activity guided fractionation of antifungal compounds by thin-layer chromatography. Besides silica gel G60 F-254 aluminium- and glass-backed plates, chemically modified silica gel (RP-18 F254S, RP-18 WF(254)s, RP-8, etc.) plates and various organic solvents were used to evaluate the compatibility of the agar spray technique. Two fungicides, Iprodione (Rovral(R)) and Mepronil (Bastak(R)), were tested to determine the sensitivity of the test fungus.

Keywords

agar spray
bioautography
thin-layer chromatography
valsa ceratosperma (tode ex fries) maire

Affiliation entities

Not a UNIGE publication

Citation (ISO format)

ISLAM, N. et al. Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents. In: Pharmaceutical biology, 2003, vol. 41, n° 8, p. 637–640. doi: 10.1080/13880200390502621

Article (Published version)

Identifiers

PID : unige:11697
DOI : 10.1080/13880200390502621

Journal ISSN1388-0209

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Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

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