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Scientific article
English

Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents

Published inPharmaceutical biology, vol. 41, no. 8, p. 637-640
Publication date2003
Abstract

A simple bioautographic agar spray assay has been developed using the plant pathogenic fungus Valsa ceratosperma as an indicator organism for the detection and activity guided fractionation of antifungal compounds by thin-layer chromatography. Besides silica gel G60 F-254 aluminium- and glass-backed plates, chemically modified silica gel (RP-18 F254S, RP-18 WF(254)s, RP-8, etc.) plates and various organic solvents were used to evaluate the compatibility of the agar spray technique. Two fungicides, Iprodione (Rovral(R)) and Mepronil (Bastak(R)), were tested to determine the sensitivity of the test fungus.

Keywords
  • agar spray
  • bioautography
  • thin-layer chromatography
  • valsa ceratosperma (tode ex fries) maire
Affiliation Not a UNIGE publication
Citation (ISO format)
ISLAM, N. et al. Bioautography with the Fungus Valsa ceratosperma in the Search for Antimycotic Agents. In: Pharmaceutical biology, 2003, vol. 41, n° 8, p. 637–640. doi: 10.1080/13880200390502621
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1388-0209
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