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Scientific article
English

On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography–UV detection using post-column derivatization and liquid chromatography–mass spectrometry

Published inJournal of chromatography, vol. 1082, no. 1, p. 51-59
Publication date2005
Abstract

Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization-MS/MS and collision-induced dissociation-MS). On-line UV and MS data demonstrated the presence of methoxyflavone glycosides and aglycones in a total of seven compounds. Three naturally occurring flavones glycosides and two unusual erythro- and threo-diastereoisomeric flavolignan 7-O-glucosides were identified together with their aglycones.

Keywords
  • Saccharum officinarum
  • Lc/uv
  • Lc/ms
  • Flavonoids
  • Transgenic plants
Citation (ISO format)
COLOMBO, Renata et al. On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography–UV detection using post-column derivatization and liquid chromatography–mass spectrometry. In: Journal of chromatography, 2005, vol. 1082, n° 1, p. 51–59. doi: 10.1016/j.chroma.2005.01.083
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ISSN of the journal0021-9673
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