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Scientific article
English

PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis

Published inMolecular biology of the cell, vol. 11, no. 9, p. 3109-3121
Publication date2000
Abstract

PECAM-1/CD31 is a cell adhesion and signaling molecule that is enriched at the endothelial cell junctions. Alternative splicing generates multiple PECAM-1 splice variants, which differ in their cytoplasmic domains. It has been suggested that the extracellular ligand-binding property, homophilic versus heterophilic, of these isoforms is controlled by their cytoplasmic tails. To determine whether the cytoplasmic domains also regulate the cell surface distribution of PECAM-1 splice variants, we examined the distribution of CD31-EGFPs (PECAM-1 isoforms tagged with the enhanced green fluorescent protein) in living Chinese hamster ovary cells and in PECAM-1-deficient endothelial cells. Our results indicate that the extracellular, rather than the cytoplasmic domain, directs PECAM-1 to the cell-cell borders. Furthermore, coculturing PECAM-1 expressing and deficient cells along with transfection of CD31-EGFP cDNAs into PECAM-1 deficient cells reveal that this PECAM-1 localization is mediated by homophilic interactions. Although the integrin alphavbeta3 has been shown to interact with PECAM-1, this trans-heterophilic interaction was not detected at the borders of endothelial cells. However, based on cocapping experiments performed on proT cells, we provide evidence that the integrin alphavbeta3 associates with PECAM-1 on the same cell surface as in a cis manner.

Keywords
  • Alternative Splicing
  • Animals
  • Antigens, CD31/chemistry/ genetics/ metabolism
  • CHO Cells
  • Capillaries/cytology/physiology
  • Cell Line
  • Cells, Cultured
  • Cerebrovascular Circulation
  • Cricetinae
  • Cytoplasm/physiology
  • Endothelium, Vascular/ physiology/ultrastructure
  • Exons
  • Green Fluorescent Proteins
  • Intercellular Junctions/ physiology
  • Luminescent Proteins/analysis
  • Mice
  • Mice, Knockout
  • Models, Molecular
  • Protein Conformation
  • Receptors, Vitronectin/chemistry/ metabolism
  • Recombinant Fusion Proteins/analysis/metabolism
  • Signal Transduction
  • Transfection
Citation (ISO format)
WONG, C. W. et al. PECAM-1/CD31 trans-homophilic binding at the intercellular junctions is independent of its cytoplasmic domain; evidence for heterophilic interaction with integrin alphavbeta3 in Cis. In: Molecular biology of the cell, 2000, vol. 11, n° 9, p. 3109–3121. doi: 10.1091/mbc.11.9.3109
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ISSN of the journal1059-1524
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