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A chimeric cell adhesion molecule mediates homing of lymphocytes to vascularized tumors

Published in Cancer Research. 1999, vol. 59, no. 20, p. 5255-5263
Abstract To facilitate tumor colonization by adoptively transferred cells of the immune system, we created a chimeric cell adhesion molecule that mediates tumor-specific homing by binding to the integrin alpha(v)beta3 on angiogenic endothelial cells. A high-affinity cell adhesion molecule for integrin alpha(v)beta3 was generated by fusing the disintegrin kistrin to the transmembrane adhesion molecule CD31/PECAM-1. This chimeric cell adhesion molecule, termed KISS31, mediates adhesion of lymphoid cells to soluble recombinant integrin alpha(v)beta3 and to endotheliomal monolayers in vitro. KISS31-expressing lymphoid cells accumulate in angiogenic tumors in two in vivo models, in B16/129 melanoma xenografts on the chick chorioallantois and in s.c. growing Lewis lung carcinoma in mice. Our data indicate that expression of KISS31 on lymphoid cells confers tumor-specific homing. This is, to our knowledge, the first example of an experimental mechanism that targets living cells to tumors by redirecting their homing pattern.
Keywords Amino Acid SequenceAnimalsAntigens, CD31/ physiologyBase SequenceCell MovementChickensImmunotherapy, AdoptiveLymphocytes/ immunology/physiologyMiceMice, Inbred C57BLMolecular Sequence DataNeoplasms, Experimental/ therapyPeptides/ physiologyReceptors, Vitronectin/physiologyRecombinant Fusion Proteins/ physiologyTumor Cells, Cultured
PMID: 10537306
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WIEDLE, Guido, JOHNSON-LEGER, Caroline, IMHOF, Beat. A chimeric cell adhesion molecule mediates homing of lymphocytes to vascularized tumors. In: Cancer Research, 1999, vol. 59, n° 20, p. 5255-5263.

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Deposited on : 2010-08-27

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