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Scientific article
English

A chimeric cell adhesion molecule mediates homing of lymphocytes to vascularized tumors

Published inCancer research, vol. 59, no. 20, p. 5255-5263
Publication date1999
Abstract

To facilitate tumor colonization by adoptively transferred cells of the immune system, we created a chimeric cell adhesion molecule that mediates tumor-specific homing by binding to the integrin alpha(v)beta3 on angiogenic endothelial cells. A high-affinity cell adhesion molecule for integrin alpha(v)beta3 was generated by fusing the disintegrin kistrin to the transmembrane adhesion molecule CD31/PECAM-1. This chimeric cell adhesion molecule, termed KISS31, mediates adhesion of lymphoid cells to soluble recombinant integrin alpha(v)beta3 and to endotheliomal monolayers in vitro. KISS31-expressing lymphoid cells accumulate in angiogenic tumors in two in vivo models, in B16/129 melanoma xenografts on the chick chorioallantois and in s.c. growing Lewis lung carcinoma in mice. Our data indicate that expression of KISS31 on lymphoid cells confers tumor-specific homing. This is, to our knowledge, the first example of an experimental mechanism that targets living cells to tumors by redirecting their homing pattern.

Keywords
  • Amino Acid Sequence
  • Animals
  • Antigens, CD31/ physiology
  • Base Sequence
  • Cell Movement
  • Chickens
  • Immunotherapy, Adoptive
  • Lymphocytes/ immunology/physiology
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Neoplasms, Experimental/ therapy
  • Peptides/ physiology
  • Receptors, Vitronectin/physiology
  • Recombinant Fusion Proteins/ physiology
  • Tumor Cells, Cultured
Affiliation Not a UNIGE publication
Citation (ISO format)
WIEDLE, Guido, JOHNSON-LEGER, Caroline, IMHOF, Beat. A chimeric cell adhesion molecule mediates homing of lymphocytes to vascularized tumors. In: Cancer research, 1999, vol. 59, n° 20, p. 5255–5263.
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ISSN of the journal0008-5472
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