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Title

Interleukin 3 perfusion prevents death due to acute anemia induced by monoclonal antierythrocyte autoantibody

Authors
Shibata, T.
Kindler, V.
Published in The Journal of experimental medicine. 1990, vol. 171, no. 5, p. 1809-1814
Abstract We have evaluated the therapeutic activity of rIL-3, in comparison with recombinant granulocyte-macrophage CSF (rGM-CSF) and recombinant erythropoietin (rEpo), on a lethal form of acute anemia induced by a single injection of a monoclonal IgG1 anti-mouse RBC (MRBC) autoantibody. Continuous perfusion of rIL-3 before the administration of anti-MRBC mAb prevented animals from the death due to anemia with a rapid recovery in greater than 90% of the cases, while only partial protection (one third of the cases) was obtained by rEpo perfusion, and no protection by rGM-CSF. Since the anti-MRBC mAb induced a marked agglutination of RBC in spleens and livers, and subsequent hemodynamic failure may be an additional contributing factor to the animals' death, the activation of Fc gamma receptor-dependent phagocytosis by rIL-3, as well as the increased number of monocytes/macrophages resulting from rIL-3 perfusion, may also facilitate rapid elimination of these agglutinated RBC, resulting in the further amelioration of the animals' survival. Our results suggest that the therapeutic effect of rIL-3 on anti-MRBC autoantibody-induced anemia is achieved by: (a) its activity to promote the growth and differentiation of erythroid progenitors responsive to Epo and of monocyte/macrophage lineage; and (b) its activity to enhance the phagocytic activity of macrophages to efficiently eliminate agglutinated RBC in spleens and livers.
Keywords Acute DiseaseAnemia/immunology/pathology/ therapyAnimalsAntibodies, MonoclonalAutoantibodies/ immunologyColony-Stimulating Factors/ therapeutic useEndocytosisErythrocytes/ immunologyErythropoietin/ therapeutic useGranulocyte-Macrophage Colony-Stimulating FactorGrowth Substances/ therapeutic useHematocritInterleukin-3/administration & dosage/ therapeutic useLiver/pathologyMiceMice, Inbred BALB CMice, Inbred StrainsPerfusionReceptors, Fc/physiologyRecombinant Proteins/administration & dosage/therapeutic useSpleen/pathology
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PMID: 2185334
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SHIBATA, T. et al. Interleukin 3 perfusion prevents death due to acute anemia induced by monoclonal antierythrocyte autoantibody. In: The Journal of experimental medicine, 1990, vol. 171, n° 5, p. 1809-1814. doi: 10.1084/jem.171.5.1809 https://archive-ouverte.unige.ch/unige:11584

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Deposited on : 2010-08-27

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