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Scientific article
English

Interleukin 3 perfusion prevents death due to acute anemia induced by monoclonal antierythrocyte autoantibody

Published inThe Journal of experimental medicine, vol. 171, no. 5, p. 1809-1814
Publication date1990
Abstract

We have evaluated the therapeutic activity of rIL-3, in comparison with recombinant granulocyte-macrophage CSF (rGM-CSF) and recombinant erythropoietin (rEpo), on a lethal form of acute anemia induced by a single injection of a monoclonal IgG1 anti-mouse RBC (MRBC) autoantibody. Continuous perfusion of rIL-3 before the administration of anti-MRBC mAb prevented animals from the death due to anemia with a rapid recovery in greater than 90% of the cases, while only partial protection (one third of the cases) was obtained by rEpo perfusion, and no protection by rGM-CSF. Since the anti-MRBC mAb induced a marked agglutination of RBC in spleens and livers, and subsequent hemodynamic failure may be an additional contributing factor to the animals' death, the activation of Fc gamma receptor-dependent phagocytosis by rIL-3, as well as the increased number of monocytes/macrophages resulting from rIL-3 perfusion, may also facilitate rapid elimination of these agglutinated RBC, resulting in the further amelioration of the animals' survival. Our results suggest that the therapeutic effect of rIL-3 on anti-MRBC autoantibody-induced anemia is achieved by: (a) its activity to promote the growth and differentiation of erythroid progenitors responsive to Epo and of monocyte/macrophage lineage; and (b) its activity to enhance the phagocytic activity of macrophages to efficiently eliminate agglutinated RBC in spleens and livers.

Keywords
  • Acute Disease
  • Anemia/immunology/pathology/ therapy
  • Animals
  • Antibodies, Monoclonal
  • Autoantibodies/ immunology
  • Colony-Stimulating Factors/ therapeutic use
  • Endocytosis
  • Erythrocytes/ immunology
  • Erythropoietin/ therapeutic use
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Growth Substances/ therapeutic use
  • Hematocrit
  • Interleukin-3/administration & dosage/ therapeutic use
  • Liver/pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred Strains
  • Perfusion
  • Receptors, Fc/physiology
  • Recombinant Proteins/administration & dosage/therapeutic use
  • Spleen/pathology
Citation (ISO format)
SHIBATA, T. et al. Interleukin 3 perfusion prevents death due to acute anemia induced by monoclonal antierythrocyte autoantibody. In: The Journal of experimental medicine, 1990, vol. 171, n° 5, p. 1809–1814. doi: 10.1084/jem.171.5.1809
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ISSN of the journal0022-1007
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