en
Scientific article
English

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published inJournal of Visualized Experiments, no. 133
Publication date2018
Abstract

B lymphocytes are key players in immune cell circulation and they mainly home to and reside in lymphoid organs. While normal B cells only proliferate when stimulated by T lymphocytes, oncogenic B cells survive and expand autonomously in undefined organ niches. Mantle cell lymphoma (MCL) is one such B cell disorder, where the median survival rate of patients is 4 - 5 years. This calls for the need of effective mechanisms by which the homing and engraftment of these cells are blocked in order to increase the survival and longevity of patients. Therefore, the effort to develop a xenograft mouse model to study the efficacy of MCL therapeutics by blocking the homing mechanism in vivo is of utmost importance. Development of animal recipients for human cell xenotransplantation to test early stage drugs have long been pursued, as relevant preclinical mouse models are crucial to screen new therapeutic agents. This animal model is developed to avoid human graft rejection and to establish a model for human diseases, and it may be an extremely useful tool to study disease progression of different lymphoma types and to perform preclinical testing of candidate drugs for hematologic malignancies, like MCL. We established a xenograft mouse model that will serve as an excellent resource to study and develop novel therapeutic approaches for MCL.

Keywords
  • Animals
  • Antineoplastic Agents/therapeutic use
  • B-Lymphocytes/metabolism
  • Cell Line, Tumor
  • Disease Models, Animal
  • Heterografts/transplantation
  • Humans
  • Lymphoma, Mantle-Cell/surgery
  • Mice
  • Mice, SCID
  • Transplantation, Heterologous
  • Xenograft Model Antitumor Assays
Citation (ISO format)
VIJAYA KUMAR, Archana Vijaya Kumar et al. Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma. In: Journal of Visualized Experiments, 2018, n° 133. doi: 10.3791/56023
Identifiers
ISSN of the journal1940-087X
325views
0downloads

Technical informations

Creation2018/11/23 15:18:00
First validation2018/11/23 15:18:00
Update time2023/03/15 16:05:44
Status update2023/03/15 16:05:44
Last indexation2024/01/17 05:16:12
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack