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Generic and context-dependent gene modulations during Hydra whole body regeneration

Year 2019
Description 27
Abstract The cnidarian Hydra is a classical model of whole-body regeneration. Historically, Hydra apical regeneration has received more attention than its basal counterpart, most studies considering these two regenerative processes independently. We present here a transcriptome-wide comparative analysis of apical and basal regeneration after decapitation and mid-gastric bisection, augmented with a characterization of positional and cell-type expression patterns in non-regenerating animals. The profiles of 25'637 Hydra transcripts are available on HydrATLAS (https://hydratlas.unige.ch), a web interface allowing a convenient access to each transcript profile. These data indicate that generic impulse-type modulations occur during the first four hours post-amputation, consistent with a similar integration of injury-related cues on both sides of the amputation plane. Initial divergences in gene regulations are observed in regenerating tips between four and eight hours post-amputation, followed by a dramatic transcriptomic reprogramming between eight and 16 hours when regulations become sustained. As expected, central components of apical patterning, Wnt3 and HyBra1, are among the earliest genes up-regulated during apical regeneration. During early basal regeneration, a BMP signaling ligand (BMP5-8c) and a potential BMP inhibitor (NBL1) are up-regulated, suggesting that BMP signaling is involved in the basal organizer, as supported by higher levels of phosphorylated Smad in the basal region and by the LiCl-induced extension of NBL1 expression. By contrast, upon ectopic activation of Wnt/b-catenin signaling, NBL1 is no longer expressed, basal differentiation is not maintained and basal regeneration is abolished. A tight cross-talk between Wnt/b-catenin apically and BMP signaling basally appears necessary for maintaining and regenerating Hydra anatomy.
Keywords RegenerationTranscriptomicsRNA-seqWnt signalingBMP signalingHydraBasal organizerApical organizer
Note Soumis dans : BioRxiv
Full text
Swiss National Science Foundation: 31003A-130337, 31003A_149630, 31003_169930)
Autre: HFSP RHP0016-2010
(ISO format)
WENGER, Yvan et al. Generic and context-dependent gene modulations during Hydra whole body regeneration. 2019. doi: 10.1101/587147 https://archive-ouverte.unige.ch/unige:115740

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Deposited on : 2019-04-04

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