Scientific Article
previous document  unige:11533  next document
add to browser collection
Title

Junctional adhesion molecule-C is a soluble mediator of angiogenesis

Authors
Rabquer, B. J.
Amin, M. A.
Teegala, Nanditha
Shaheen, M. K.
Tsou, P. S.
Ruth, J. H.
Lesch, C. A.
show hidden authors show all authors [1 - 9]
Published in Journal of Immunology. 2010, vol. 185, no. 3, p. 1777-1785
Abstract Junctional adhesion molecule-C (JAM-C) is an adhesion molecule expressed by endothelial cells (ECs) that plays a role in tight junction formation, leukocyte adhesion, and transendothelial migration. In the current study, we investigated whether JAM-C is found in soluble form and whether soluble JAM-C (sJAM-C) mediates angiogenesis. We found that JAM-C is present in soluble form in normal serum and elevated in rheumatoid arthritis (RA) serum. The concentration of sJAM-C is also elevated locally in RA synovial fluid compared with RA serum or osteoarthritis synovial fluid. sJAM-C was also present in the culture supernatant of human microvascular ECs (HMVECs) and immortalized human dermal microvascular ECs, and its concentration was increased following cytokine stimulation. In addition, sJAM-C cleavage from the cell surface was mediated in part by a disintegrin and metalloproteinases 10 and 17. In functional assays, sJAM-C was both chemotactic and chemokinetic for HMVECs and induced HMVEC tube formation on Matrigel in vitro. Neutralizing anti-JAM-C Abs inhibited RA synovial fluid-induced HMVEC chemotaxis and sJAM-C-induced HMVEC tube formation on Matrigel. sJAM-C also induced angiogenesis in vivo in the Matrigel plug and sponge granuloma models. Moreover, sJAM-C-mediated HMVEC chemotaxis was dependent on Src, p38, and PI3K. Our results show that JAM-C exists in soluble form and suggest that modulation of sJAM-C may provide a novel route for controlling pathological angiogenesis.
Identifiers
PMID: 20592283
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://www.jimmunol.org/cgi/reprint/185/3/1777.pdf
Citation
(ISO format)
RABQUER, B. J. et al. Junctional adhesion molecule-C is a soluble mediator of angiogenesis. In: Journal of Immunology, 2010, vol. 185, n° 3, p. 1777-1785. https://archive-ouverte.unige.ch/unige:11533

107 hits

0 download

Update

Deposited on : 2010-08-27

Export document
Format :
Citation style :