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Scientific article
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Scanning force microscopy and cryo-electron microscopy of tobacco mosaic virus as a test specimen

Published inUltramicroscopy, vol. 42-44, p. 1168-1172
Publication date1992
Abstract

In this study, tobacco mosaic virus (TMV) provides a resolution criterion for specimen preparation methods as well as for imaging parameters of the scanning force microscope (SFM). We present scanning force microscopic images of the virus embedded in 0.5% buffered phosphotungstic acid solution adsorbed on a freshly cleaved mica surface, and imaged under atmospheric conditions. Individual TMV particles were clearly identified with a characteristic shape of long rods of about 300 nm long and 60–70 nm in apparent width due to the geometric parameters of the tip. The structure of the virus was compared with cryo-electron microscopic data of vitrified suspensions observed to a resolution of 1.15 nm. Uncoated TMV particles were also deposited on evaporated titanium thin films and imaged by SFM.

Citation (ISO format)
ZENHAUSERN, Frédéric et al. Scanning force microscopy and cryo-electron microscopy of tobacco mosaic virus as a test specimen. In: Ultramicroscopy, 1992, vol. 42-44, p. 1168–1172. doi: 10.1016/0304-3991(92)90419-K
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ISSN of the journal0304-3991
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