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Scientific article
English

Scanning force microscopy and cryo-electron microscopy of tobacco mosaic virus as a test specimen

Contributors

Zenhausern, Frédéric; Adrian, M.; Emch, Roger; Taborelli, Mauro; Jobin, Marc; Descouts, Pierre

Published in

Ultramicroscopy, vol. 42-44, p. 1168-1172

Publication date

1992

Abstract

In this study, tobacco mosaic virus (TMV) provides a resolution criterion for specimen preparation methods as well as for imaging parameters of the scanning force microscope (SFM). We present scanning force microscopic images of the virus embedded in 0.5% buffered phosphotungstic acid solution adsorbed on a freshly cleaved mica surface, and imaged under atmospheric conditions. Individual TMV particles were clearly identified with a characteristic shape of long rods of about 300 nm long and 60–70 nm in apparent width due to the geometric parameters of the tip. The structure of the virus was compared with cryo-electron microscopic data of vitrified suspensions observed to a resolution of 1.15 nm. Uncoated TMV particles were also deposited on evaporated titanium thin films and imaged by SFM.

UNIGE affiliation entities

Citation (ISO format)

ZENHAUSERN, Frédéric et al. Scanning force microscopy and cryo-electron microscopy of tobacco mosaic virus as a test specimen. In: Ultramicroscopy, 1992, vol. 42-44, p. 1168–1172. doi: 10.1016/0304-3991(92)90419-K

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Article (Published version)

Identifiers

Additional URL for this publication

http://linkinghub.elsevier.com/retrieve/pii/030439919290419K

Journal ISSN

0304-3991
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Technical informations

Creation

20/03/2019 12:46:00

First validation

20/03/2019 12:46:00

Update

15/03/2023 16:01:52

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15/03/2023 16:01:51

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