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Scientific article
English

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood

Published inXenotransplantation, vol. 25, no. 1
Publication date2018
Abstract

In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E (HLA-E) on pEC. Thus, the aim of this study was to analyze NK cell responses to vascularized xenografts using an ex vivo perfusion system of pig limbs with human blood.

Keywords
  • Animals
  • Animals, Genetically Modified/immunology
  • Cytotoxicity, Immunologic/immunology
  • Endothelial Cells/immunology
  • Extremities/blood supply
  • HLA Antigens/genetics
  • Heterografts/immunology
  • Histocompatibility Antigens Class I/genetics
  • Humans
  • Killer Cells, Natural/immunology
  • Leukocytes/metabolism
  • Leukocytes, Mononuclear/immunology
  • Membrane Cofactor Protein/genetics/immunology
  • Transplantation, Heterologous/methods
Citation (ISO format)
PUGA YUNG, Gisella et al. Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood. In: Xenotransplantation, 2018, vol. 25, n° 1. doi: 10.1111/xen.12357
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0908-665X
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Creation10/24/2018 2:20:00 PM
First validation10/24/2018 2:20:00 PM
Update time03/15/2023 4:01:39 PM
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