Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood

Puga Yung, Gisella; Bongoni, Anjan K; Pradier, Amandine; Madelon, Natacha; Papaserafeim, Maria; Sfriso, Riccardo; Ayares, David L; Wolf, Eckhard; Klymiuk, Nikolai; Bähr, Andrea; Constantinescu, Mihai A; Voegelin, Esther; Kiermeir, David; Jenni, Hansjörg; Rieben, Robert; Seebach, Jorg Dieter

doi:10.1111/xen.12357

Scientific article

English

Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood

ContributorsPuga Yung, Gisella

; Bongoni, Anjan K; Pradier, Amandine; Madelon, Natacha; Papaserafeim, Maria; Sfriso, Riccardo; Ayares, David L; Wolf, Eckhard; Klymiuk, Nikolai; Bähr, Andrea; Constantinescu, Mihai A; Voegelin, Esther; Kiermeir, David; Jenni, Hansjörg; Rieben, Robert; Seebach, Jorg Dieter

Published inXenotransplantation, vol. 25, no. 1

Publication date2018

Abstract

In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E (HLA-E) on pEC. Thus, the aim of this study was to analyze NK cell responses to vascularized xenografts using an ex vivo perfusion system of pig limbs with human blood.

Keywords

Animals
Animals, Genetically Modified/immunology
Cytotoxicity, Immunologic/immunology
Endothelial Cells/immunology
Extremities/blood supply
HLA Antigens/genetics
Heterografts/immunology
Histocompatibility Antigens Class I/genetics
Humans
Killer Cells, Natural/immunology
Leukocytes/metabolism
Leukocytes, Mononuclear/immunology
Membrane Cofactor Protein/genetics/immunology
Transplantation, Heterologous/methods

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de médecine

Research groups

Cellules NK et récepteurs FcyR dans l'auto-immunité et le rejet de xénotransplantation (856)

Citation (ISO format)

PUGA YUNG, Gisella et al. Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood. In: Xenotransplantation, 2018, vol. 25, n° 1. doi: 10.1111/xen.12357

Article (Published version)

Identifiers

PID : unige:115267
DOI : 10.1111/xen.12357
PMID : 29057510

Journal ISSN0908-665X

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Creation24/10/2018 14:20:00

First validation24/10/2018 14:20:00

Update09/01/2025 10:27:10

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Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood

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