Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood

Abicht, Jan-Michael; Sfriso, Riccardo; Reichart, Bruno; Längin, Matthias; Gahle, Katja; Puga Yung, Gisella; Seebach, Jorg Dieter; Rieben, Robert; Ayares, David; Wolf, Eckhard; Klymiuk, Nikolai; Baehr, Andrea; Kind, Alexander; Mayr, Tanja; Bauer, Andreas

doi:10.1111/xen.12390

Scientific article

English

Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood

ContributorsAbicht, Jan-Michael; Sfriso, Riccardo; Reichart, Bruno; Längin, Matthias; Gahle, Katja; Puga Yung, Gisella; Seebach, Jorg Dieter; Rieben, Robert; Ayares, David; Wolf, Eckhard; Klymiuk, Nikolai; Baehr, Andrea; Kind, Alexander; Mayr, Tanja; Bauer, Andreas

Published inXenotransplantation, vol. 25, no. 5, e12390

Publication date2018

Abstract

In pig-to-human xenotransplantation, early cellular rejection reactions are mediated by natural killer cells (NK cells). Human NK cells are inhibited by HLA-E via CD94/NKG2A receptors. To protect porcine grafts against human NK cell responses, transgenic GTKO pigs expressing hCD46 and HLA-E have been generated. The aim of this study was to test the effect of this genetic modification on xenogeneic, and in particular human NK cell response, using an ex vivo perfusion model of pig hearts with human blood.

Affiliation

Faculté de médecine / Section de médecine clinique / Département de médecine

Research group

Laboratoire d'immunologie translationnelle (856)

Citation (ISO format)

ABICHT, Jan-Michael et al. Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood. In: Xenotransplantation, 2018, vol. 25, n° 5, p. e12390. doi: 10.1111/xen.12390

Article (Published version)

Identifiers

PID : unige:115263
DOI : 10.1111/xen.12390
PMID : 29536572

ISSN of the journal0908-665X

380views

0downloads

Creation10/24/2018 2:16:00 PM

First validation10/24/2018 2:16:00 PM

Update time03/15/2023 4:01:35 PM

Status update03/15/2023 4:01:34 PM

Last indexation01/17/2024 5:09:47 AM

Archive ouverte UNIGE

Multiple genetically modified GTKO/hCD46/HLA-E/hβ2-mg porcine hearts are protected from complement activation and natural killer cell infiltration during ex vivo perfusion with human blood

Technical informations