A global HILIC-MS approach to measure polar human cerebrospinal fluid metabolome: Exploring gender-associated variation in a cohort of elderly cognitively healthy subjects

Gallart-Ayala, Héctor; Konz, Ioana; Mehl, Florence; Teav, Tony; Oikonomidi, Aikaterini; Peyratout, Gwendoline; van der Velpen, Vera; Popp, Julius; Ivanisevic, Julijana

Advanced search

Browse by...

Deposit

Highlights

More informations

Title		A global HILIC-MS approach to measure polar human cerebrospinal fluid metabolome: Exploring gender-associated variation in a cohort of elderly cognitively healthy subjects
Authors		Gallart-Ayala, Héctor Konz, Ioana Mehl, Florence Teav, Tony Oikonomidi, Aikaterini Peyratout, Gwendoline van der Velpen, Vera Popp, Julius Ivanisevic, Julijana show all authors [1 - 9]
Published in		Analytica Chimica Acta. 2018, vol. 1037, p. 327-337
Abstract		Cerebrospinal fluid (CSF) is a key body fluid that maintains the homeostasis in central nervous system (CNS). As a biofluid whose content reflects the brain metabolic activity, the CSF has been profiled in the context of neurological diseases to provide novel insights into the disease mechanisms. However, a global high-throughput approach to measure a broad diversity of polar metabolites present in CSF is lacking. Although still perceived as challenging and less reproducible, hydrophilic interaction liquid chromatography (HILIC) has recently evolved to offer the unprecedented coverage capacity of water-soluble metabolome. Here, we present a global HILIC high-resolution mass spectrometry-based (HRMS) approach that combines the profiling in acidic pH ESI (+) and basic pH ESI (-) mode to extend the coverage of CSF polar metabolome. This approach allowed us to annotate and measure a broad range of central carbon metabolites (implicated in glycolysis, TCA cycle, nucleotide, amino acid and fatty acid metabolism) in CSF collected from cognitively healthy elderly volunteers (n = 32), using a single extraction method. Metabolite annotation was achieved using the accurate mass, RT and MS/MS criteria, allowing for the characterization of 146 measurable metabolites. Exploration of characterized individual CSF profiles allowed for a discovery of intriguing gender-associated differences, with significantly higher acylcarnitine levels in men and higher taurine levels women. With this case study, we demonstrate the value of combined HILIC ESI ± HRMS profiling to assess CSF metabolome in clinical research studies.
Identifiers		DOI: 10.1016/j.aca.2018.04.002 PMID: 30292309
Full text		Article (Published version) (2.4 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de psychiatrie
Research group		Neuropsychiatrie et Biomarqueurs de la maladie d'Alzheimer (991)
Citation (ISO format)		GALLART-AYALA, Héctor et al. A global HILIC-MS approach to measure polar human cerebrospinal fluid metabolome: Exploring gender-associated variation in a cohort of elderly cognitively healthy subjects. In: Analytica Chimica Acta, 2018, vol. 1037, p. 327-337. https://archive-ouverte.unige.ch/unige:115254