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Diselenolane‐Mediated Cellular Uptake: Efficient Cytosolic Delivery of Probes, Peptides, Proteins, Artificial Metalloenzymes and Protein‐Coated Quantum Dots

Publié dansChemistry - A European Journal, vol. 25, no. 16, p. 4047-4051
Date de publication2019
Résumé

Cyclic oligochalcogenides are emerging as powerful tools to penetrate cells. With disulfide ring tension maximized, selenium chemistry had to be explored next to enhance speed and selectivity of dynamic covalent exchange on the way into the cytosol. We show that diseleno lipoic acid (DiSeL) delivers a variety of relevant substrates. DiSeL‐driven uptake of artificial metalloenzymes enables bioorthogonal fluorophore uncaging within cells. Binding of a bicyclic peptide, phalloidin, to actin fibers evinces targeted delivery to the cytosol. Automated tracking of diffusive compared to directed motility and immobility localizes 79 % of protein‐coated quantum dots (QDs) in the cytosol, with little endosomal capture (0.06 %). These results suggest that diselenolanes might act as molecular walkers along disulfide tracks in locally denatured membrane proteins, surrounded by adaptive micellar membrane defects. Miniscule and versatile, DiSeL tags are also readily available, stable, soluble, and non‐toxic.

Citation (format ISO)
BARTOLAMI, Eline et al. Diselenolane‐Mediated Cellular Uptake: Efficient Cytosolic Delivery of Probes, Peptides, Proteins, Artificial Metalloenzymes and Protein‐Coated Quantum Dots. In: Chemistry - A European Journal, 2019, vol. 25, n° 16, p. 4047–4051. doi: 10.1002/chem.201805900
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ISSN du journal0947-6539
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Informations techniques

Création19/03/2019 17:22:00
Première validation19/03/2019 17:22:00
Heure de mise à jour15/03/2023 16:00:14
Changement de statut15/03/2023 16:00:14
Dernière indexation17/01/2024 05:07:35
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