Multi-scale X-ray computed tomography to detect and localize metal-based nanomaterials in lung tissues of in vivo exposed mice

Chaurand, Perrine; Liu, Wei; Borschneck, Daniel; Levard, Clément; Auffan, Mélanie; Paul, Emmanuel; Collin, Blanche; Kieffer, Isabelle; Lanone, Sophie; Rose, Jérôme; Perrin, Jeanne

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Title		Multi-scale X-ray computed tomography to detect and localize metal-based nanomaterials in lung tissues of in vivo exposed mice
Authors		Chaurand, Perrine Liu, Wei Borschneck, Daniel Levard, Clément Auffan, Mélanie Paul, Emmanuel Collin, Blanche Kieffer, Isabelle Lanone, Sophie Rose, Jérôme Perrin, Jeanne show all authors [1 - 11]
Published in		Scientific Reports. 2018, vol. 8, no. 1, p. 4408
Abstract		In this methodological study, we demonstrated the relevance of 3D imaging performed at various scales for the ex vivo detection and location of cerium oxide nanomaterials (CeO2-NMs) in mouse lung. X-ray micro-computed tomography (micro-CT) with a voxel size from 14 µm to 1 µm (micro-CT) was combined with X-ray nano-computed tomography with a voxel size of 63 nm (nano-CT). An optimized protocol was proposed to facilitate the sample preparation, to minimize the experimental artifacts and to optimize the contrast of soft tissues exposed to metal-based nanomaterials (NMs). 3D imaging of the NMs biodistribution in lung tissues was consolidated by combining a vast variety of techniques in a correlative approach: histological observations, 2D chemical mapping and speciation analysis were performed for an unambiguous detection of NMs. This original methodological approach was developed following a worst-case scenario of exposure, i.e. high dose of exposure with administration via intra-tracheal instillation. Results highlighted both (i) the non-uniform distribution of CeO2-NMs within the entire lung lobe (using large field-of-view micro-CT) and (ii) the detection of CeO2-NMs down to the individual cell scale, e.g. macrophage scale (using nano-CT with a voxel size of 63 nm).
Identifiers		DOI: 10.1038/s41598-018-21862-4 PMID: 29535369
Full text		Article (Published version) (3.2 MB) - Free access
Citation (ISO format)		CHAURAND, Perrine et al. Multi-scale X-ray computed tomography to detect and localize metal-based nanomaterials in lung tissues of in vivo exposed mice. In: Scientific Reports, 2018, vol. 8, n° 1, p. 4408. https://archive-ouverte.unige.ch/unige:115094