Scientific article

Expression of aberrant forms of CD22 on B lymphocytes in Cd22a lupus-prone mice affects ligand binding

Published inInternational immunology, vol. 18, no. 1, p. 59-68
Publication date2006

CD22 functions primarily as a negative regulator of B-cell receptor signaling. The Cd22a allele has been proposed as a candidate allele for murine systemic lupus erythematosus. In this study, we explored the possible expression of aberrant forms of CD22, which differ in the N-terminal sequences constituting the ligand-binding site due to synthesis of abnormally processed Cd22 mRNA, in several Cd22a mouse strains, including C57BL/6 Cd22 congenic mice. The staining pattern of splenic B cells obtained with CY34 anti-CD22 mAb, which was expected to bind poorly to the aberrant CD22, was more heterogeneous in Cd22(a) mice than in Cd22b mice. Moreover, CD22 detected on B cells of Cd22a mice was expressed more weakly and as a smaller-sized protein, compared with Cd22b mice. Significantly, analysis with a synthetic CD22 ligand demonstrated that Cd22a mice carried a larger proportion of CD22 that was not bound by cis ligands on the B-cell surface than Cd22b mice. Finally, the study of C57BL/6 Cd22 congenic mice revealed that Cd22a B cells displayed a phenotype reminiscent of constitutively activated B cells (reduced surface IgM expression and augmented MHC class II expression), as reported for B cells expressing a mutant CD22 lacking the ligand-binding domain. Our demonstration that Cd22a B cells express aberrant forms of CD22, which can potentially deregulate B-cell signaling because of their decreased ligand-binding capacity, provides further support for Cd22a as a potential candidate allele for murine systemic lupus erythematosus.

  • Alleles
  • Animals
  • Antigens, CD22/genetics/*immunology
  • B-Lymphocytes/*immunology
  • Gene Expression Regulation/genetics/*immunology
  • Genes, MHC Class II/genetics/immunology
  • Immunoglobulin M/genetics/immunology
  • Ligands
  • Lupus Erythematosus, Systemic/genetics/immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred NZB
  • Mice, Knockout
  • RNA Processing, Post-Transcriptional/genetics/*immunology
  • Signal Transduction/genetics/*immunology
Citation (ISO format)
NITSCHKE, Lars et al. Expression of aberrant forms of CD22 on B lymphocytes in Cd22a lupus-prone mice affects ligand binding. In: International immunology, 2006, vol. 18, n° 1, p. 59–68. doi: 10.1093/intimm/dxh349
Main files (1)
ISSN of the journal0953-8178

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