en
Scientific article
English

Effect of long-term anti-CD4 or anti-CD8 treatment on the development of lpr CD4- CD8- double negative T cells and of the autoimmune syndrome in MRL-lpr/lpr mice

Published inJournal of autoimmunity, vol. 8, no. 1, p. 33-45
Publication date1995
Abstract

We have determined the effect of anti-CD4 or anti-CD8 monoclonal antibody (mAb) treatment from birth on the generation of the lpr CD4- CD8- double-negative (DN) T cell subset and on the development of lupus-like autoimmune syndrome in MRL-lpr/lpr mice. Both anti-CD4 and anti-CD8 mAb treatments resulted in a marked inhibition of lymph-adenopathy, whereas the development of the lpr DN T cells and of the lupus-like autoimmune syndrome strikingly differed in these two groups of mice. The treatment with anti-CD8 mAb almost completely blocked the appearance of the lpr DN T cells without any significant effect on the development of lupus-like autoimmune syndrome in MRL-lpr/lpr mice. In contrast, mice treated with anti-CD4 mAb failed to develop a lupus-like syndrome, while they still developed the lpr DN T cell subset, the predominant population in their lymph nodes, although absolute numbers were markedly diminished. Our results support the idea that CD8+ T cells are a major source of the lpr DN T cells, and that the lpr DN T cells play a minor, if any, role in the pathogenesis of lupus-like autoimmune syndrome in MRL-lpr/lpr mice.

Keywords
  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD4/ immunology
  • Antigens, CD8/ immunology
  • Autoimmune Diseases/ immunology/pathology
  • Blotting, Northern
  • Cell Differentiation/immunology
  • Flow Cytometry
  • Lupus Erythematosus, Systemic/immunology
  • Lymph Nodes/pathology
  • Mice
  • Mice, Mutant Strains
  • T-Lymphocyte Subsets/ immunology
Affiliation Not a UNIGE publication
Citation (ISO format)
MERINO, Ramon et al. Effect of long-term anti-CD4 or anti-CD8 treatment on the development of lpr CD4- CD8- double negative T cells and of the autoimmune syndrome in MRL-lpr/lpr mice. In: Journal of autoimmunity, 1995, vol. 8, n° 1, p. 33–45. doi: 10.1006/jaut.1995.0003
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ISSN of the journal0896-8411
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