

Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212528/pdf/98-0183.pdf
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Urokinase receptor (CD87) regulates leukocyte recruitment via beta 2 integrins in vivo |
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Published in | The Journal of experimental medicine. 1998, vol. 188, no. 6, p. 1029-1037 | |
Abstract | The urokinase receptor (CD87; uPAR) is found in close association with beta 2 integrins on leukocytes. We studied the functional consequence of this association for leukocyte adhesion and migration. In vivo, the beta 2 integrin-dependent recruitment of leukocytes to the inflamed peritoneum of uPAR-deficient mice was significantly reduced as compared with wild-type animals. In vitro, beta 2 integrin-mediated adhesion of leukocytes to endothelium was lost upon removal of uPAR from the leukocyte surface by phosphatidyl-inositol-specific phospholipase C. Leukocyte adhesion was reconstituted when soluble intact uPAR, but not a truncated form lacking the uPA-binding domain, was allowed to reassociate with the cell surface. uPAR ligation with a monoclonal antibody induced adhesion of monocytic cells and neutrophils to vascular endothelium by six- to eightfold, whereas ligation with inactivated uPA significantly reduced cell-to-cell adhesion irrespective of the beta 2 integrin-stimulating pathway. These data indicate that beta 2 integrin-mediated leukocyte-endothelial cell interactions and recruitment to inflamed areas require the presence of uPAR and define a new phenotype for uPAR-deficient mice. Moreover, uPAR ligation differentially modulates leukocyte adhesion to endothelium and provides novel targets for therapeutic strategies in inflammation-related vascular pathologies. | |
Keywords | Animals — Antibodies, Monoclonal/pharmacology — Antigens, CD18/metabolism/ physiology — Cell Adhesion/immunology — Cell Movement/ immunology — Endothelium, Vascular/cytology/immunology — Female — Humans — Leukocytes/enzymology/ immunology/metabolism — Mice — Mice, Knockout — Muscle, Smooth/cytology/immunology — Plasminogen Activators/ metabolism — Receptors, Cell Surface/genetics/immunology/ physiology — Receptors, Urokinase Plasminogen Activator — Tumor Cells, Cultured — Umbilical Veins — Urokinase-Type Plasminogen Activator/ metabolism | |
Identifiers | PMID: 9743521 | |
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![]() ![]() Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2212528/pdf/98-0183.pdf |
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Citation (ISO format) | MAY, A. E. et al. Urokinase receptor (CD87) regulates leukocyte recruitment via beta 2 integrins in vivo. In: The Journal of experimental medicine, 1998, vol. 188, n° 6, p. 1029-1037. doi: 10.1084/jem.188.6.1029 https://archive-ouverte.unige.ch/unige:11455 |