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Scientific article
English

Functional CD8+ but not CD4+ T cell responses develop independent of thymic epithelial MHC

Publication date2006
Abstract

The role of nonthymic epithelial (non-TE) MHC in T cell repertoire selection remains controversial. To analyze the relative roles of thymic epithelial (TE) and non-TE MHC in T cell repertoire selection, we have generated tetraparental aggregation chimeras (B6-nude<=>BALB/c and B6<=>BALB/c-nude) harboring T and B cells from both parents, whereas TE cells originated exclusively from the non-nude donor. These chimeras mounted protective virus-specific TE and non-TE MHC-restricted T cell responses. To further evaluate whether non-TE MHC alone was sufficient to generate a functional T cell repertoire, we generated tetraparental aggregation chimeras lacking MHC class II (B6-nude<=>MHCII(-/-)) or both MHC molecules (B6-nude<=>MHCI(-/-)II(-/-)) on TE cells, but not on cells of B6-nude origin. Chimeras with MHC-deficient TE cells mounted functional virus-specific CD8+ but not CD4+ T cell responses. Thus, maturation of functional CD4+ T cell responses required MHC class II on thymic epithelium, whereas CD8+ T cells matured in the absence of TE MHC.

Keywords
  • Animals
  • B-Lymphocytes/immunology
  • CD4-Positive T-Lymphocytes/ immunology
  • CD8-Positive T-Lymphocytes/ immunology
  • Chimera/immunology
  • Epithelial Cells/ immunology
  • Histocompatibility Antigens Class II/ immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Nude
  • Thymus Gland/ immunology
  • Viruses/immunology
Affiliation Not a UNIGE publication
Citation (ISO format)
MARTINIC, M. M. et al. Functional CD8+ but not CD4+ T cell responses develop independent of thymic epithelial MHC. In: Proceedings of the National Academy of Sciences of the United States of America, 2006, vol. 103, n° 39, p. 14435–14440. doi: 10.1073/pnas.0606707103
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Identifiers
ISSN of the journal0027-8424
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