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Deregulation of the SecYEG translocation channel upon removal of the plug domain

Maillard, A. P.
Lalani, Shifana
Duong, Franck
Published in Journal of Biological Chemistry. 2007, vol. 282, no. 2, p. 1281-1287
Abstract Previous studies have shown that the SecY plug is displaced from the center of the SecYEG channel during polypeptide translocation. The structural and functional consequences of the deletion of the plug are now examined. Both in vivo and in vitro observations indicate that the plug domain is not essential to the function of the translocon. In fact, deletion of the plug confers to the cell and to the membranes a Prl-like phenotype: reduced proton-motive force dependence of translocation, increased membrane insertion of SecA, diminished requirement for functional leader peptide, and weakened SecYEG subunit association. Although the plug domain does not seem essential, locking the plug in the center of the channel inactivates the translocon. Thus, the SecY plug is important to regulate the activity of the channel and to confer specificity to the translocation reaction. We propose that the plug contributes to the gating mechanism of the channel by maintaining the structure of the SecYEG complex in a compact closed state.
Keywords CrystallographyEscherichia coli/ metabolismEscherichia coli Proteins/ chemistry/ metabolismMembrane Proteins/chemistry/metabolismMultiprotein Complexes/ chemistry/ metabolismProtein Structure, QuaternaryProtein Structure, SecondaryProtein Structure, TertiaryProtein Transport/physiology
PMID: 17092931
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MAILLARD, A. P. et al. Deregulation of the SecYEG translocation channel upon removal of the plug domain. In: Journal of Biological Chemistry, 2007, vol. 282, n° 2, p. 1281-1287. doi: 10.1074/jbc.M610060200

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Deposited on : 2010-08-27

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