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Scientific article
English

Variability of the inhibition by total immunoglobulin of in vitro autoantibody-mediated erythrophagocytosis by mouse macrophages

Published inClinical and experimental immunology, vol. 145, no. 1, p. 155-161
Publication date2006
Abstract

Several autoimmune diseases, mainly autoantibody-mediated, are attenuated by infusion of total IgG (IVIg). The efficacy varies widely from one patient to another. Using an experimental model of in vitro phagocytosis of autoantibody-coated erythrocytes by mouse macrophages, we analysed the possible causes for such a variability. Our results indicated that the efficacy of the phagocytosis inhibition depends upon different factors, such as the isotype and the extent of polymerization of the immunoglobulin used for the treatment as well as the genetic background of the mice and the state of macrophage activation that can be influenced by concomitant viral infection. The development of an in vitro assay for the phagocytic activity of macrophages might improve the selection of patients susceptible to benefit from IVIg treatment.

Keywords
  • Animals
  • Autoantibodies/*immunology
  • Autoimmune Diseases/immunology/*therapy
  • Cells, Cultured
  • Erythrocytes/*pathology
  • Female
  • Immunoglobulin Fc Fragments/administration & dosage
  • Immunoglobulin G/administration & dosage
  • Immunoglobulin Isotypes/*administration & dosage
  • Immunoglobulins, Intravenous/*administration & dosage
  • Macrophages, Peritoneal/*physiology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Models, Animal
  • Phagocytosis
  • Polymers
  • Species Specificity
Affiliation Not a UNIGE publication
Citation (ISO format)
LEONARD, S. et al. Variability of the inhibition by total immunoglobulin of in vitro autoantibody-mediated erythrophagocytosis by mouse macrophages. In: Clinical and experimental immunology, 2006, vol. 145, n° 1, p. 155–161. doi: 10.1111/j.1365-2249.2006.03117.x
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ISSN of the journal0009-9104
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