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Scientific article
English

Interferon-beta induces brain-derived neurotrophic factor in peripheral blood mononuclear cells of multiple sclerosis patients

Published inJournal of neuroimmunology, vol. 197, no. 2, p. 147-151
Publication date2008
Abstract

Interferon-beta (IFN-beta) achieves its beneficial effect on multiple sclerosis (MS) via anti-inflammatory properties. In this study, we assessed the expression of the brain-derived neurotrophic factor (BDNF) in peripheral blood mononuclear cells (PBMC) from relapsing-remitting multiple sclerosis (RRMS) patients treated or not with IFN-beta. Intracellular BDNF was measured by Western blot and ELISA and compared with serum BDNF. We found higher levels of BDNF in PBMC of IFN-beta-treated versus non-treated patients, whereas serum levels of BDNF were similar. We hypothesize that the increased intracellular BDNF secondary to IFN-beta is not released in the periphery. This release is probably not tissue specific but in MS patients, BDNF could be specifically delivered by PBMC at the site of re-activation, i.e. within the central nervous system.

Keywords
  • Adult
  • Blotting, Western/methods
  • Brain-Derived Neurotrophic Factor/metabolism
  • Case-Control Studies
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay/methods
  • Female
  • Gene Expression Regulation/drug effects
  • Humans
  • Immunologic Factors/pharmacology
  • Interferon-beta/pharmacology
  • Leukocytes, Mononuclear/drug effects
  • Male
  • Middle Aged
  • Multiple Sclerosis/pathology
Citation (ISO format)
LALIVE D’EPINAY, Patrice et al. Interferon-beta induces brain-derived neurotrophic factor in peripheral blood mononuclear cells of multiple sclerosis patients. In: Journal of neuroimmunology, 2008, vol. 197, n° 2, p. 147–151. doi: 10.1016/j.jneuroim.2008.04.033
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Article (Accepted version)
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ISSN of the journal0165-5728
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