

Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1237098/pdf/00164992.pdf
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Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion |
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Published in | Molecular Biology of the Cell. 2005, vol. 16, no. 10, p. 4992-5003 | |
Abstract | The junctional adhesion molecules (JAMs) have been recently described as interendothelial junctional molecules and as integrin ligands. Here we show that JAM-B and JAM-C undergo heterophilic interaction in cell-cell contacts and that JAM-C is recruited and stabilized in junctional complexes by JAM-B. In addition, soluble JAM-B dissociates soluble JAM-C homodimers to form JAM-B/JAM-C heterodimers. This suggests that the affinity of JAM-C monomers to form dimers is higher for JAM-B than for JAM-C. Using antibodies against JAM-C, the formation of JAM-B/JAM-C heterodimers can be abolished. This liberates JAM-C from its vascular binding partner JAM-B and makes it available on the apical side of vessels for interaction with its leukocyte counter-receptor alpha(M)beta2 integrin. We demonstrate that the modulation of JAM-C localization in junctional complexes is a new regulatory mechanism for alpha(M)beta2-dependent adhesion of leukocytes. | |
Keywords | Animals — Cell Adhesion/ physiology — Cell Adhesion Molecules/genetics/ metabolism — Cell Line — Coculture Techniques — Cricetinae — Cricetulus — Endothelium/physiology/ultrastructure — Green Fluorescent Proteins/genetics — Humans — Immunoglobulins/genetics/ metabolism — Intercellular Junctions/ physiology/ultrastructure — Leukocytes/ physiology/ultrastructure — Macrophage-1 Antigen/ metabolism — Mice — Mice, Inbred C57BL — Microscopy, Immunoelectron — Peptides/genetics — Recombinant Fusion Proteins/genetics/metabolism | |
Identifiers | PMID: 16093349 | |
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![]() ![]() Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1237098/pdf/00164992.pdf |
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Citation (ISO format) | LAMAGNA, Chrystelle et al. Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion. In: Molecular Biology of the Cell, 2005, vol. 16, n° 10, p. 4992-5003. doi: 10.1091/mbc.E05-04-0310 https://archive-ouverte.unige.ch/unige:11407 |