Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion

Lamagna, Chrystelle; Meda, Paolo; Mandicourt, Guillaume; Brown, James; Gilbert, R. J.; Jones, E. Y.; Kiefer, Friedemann; Ruga, Pilar; Imhof, Beat; Aurrand-Lions, Michel

doi:10.1091/mbc.E05-04-0310

Advanced search

Browse by...

Deposit

Highlights

More informations

Title		Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion
Authors		Lamagna, Chrystelle Meda, Paolo Mandicourt, Guillaume Brown, James Gilbert, R. J. Jones, E. Y. Kiefer, Friedemann Ruga, Pilar Imhof, Beat Aurrand-Lions, Michel show all authors [1 - 10]
Published in		Molecular Biology of the Cell. 2005, vol. 16, no. 10, p. 4992-5003
Abstract		The junctional adhesion molecules (JAMs) have been recently described as interendothelial junctional molecules and as integrin ligands. Here we show that JAM-B and JAM-C undergo heterophilic interaction in cell-cell contacts and that JAM-C is recruited and stabilized in junctional complexes by JAM-B. In addition, soluble JAM-B dissociates soluble JAM-C homodimers to form JAM-B/JAM-C heterodimers. This suggests that the affinity of JAM-C monomers to form dimers is higher for JAM-B than for JAM-C. Using antibodies against JAM-C, the formation of JAM-B/JAM-C heterodimers can be abolished. This liberates JAM-C from its vascular binding partner JAM-B and makes it available on the apical side of vessels for interaction with its leukocyte counter-receptor alpha(M)beta2 integrin. We demonstrate that the modulation of JAM-C localization in junctional complexes is a new regulatory mechanism for alpha(M)beta2-dependent adhesion of leukocytes.
Keywords		Animals — Cell Adhesion/ physiology — Cell Adhesion Molecules/genetics/ metabolism — Cell Line — Coculture Techniques — Cricetinae — Cricetulus — Endothelium/physiology/ultrastructure — Green Fluorescent Proteins/genetics — Humans — Immunoglobulins/genetics/ metabolism — Intercellular Junctions/ physiology/ultrastructure — Leukocytes/ physiology/ultrastructure — Macrophage-1 Antigen/ metabolism — Mice — Mice, Inbred C57BL — Microscopy, Immunoelectron — Peptides/genetics — Recombinant Fusion Proteins/genetics/metabolism
Identifiers		DOI: 10.1091/mbc.E05-04-0310 PMID: 16093349
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1237098/pdf/00164992.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie
Citation (ISO format)		LAMAGNA, Chrystelle et al. Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion. In: Molecular Biology of the Cell, 2005, vol. 16, n° 10, p. 4992-5003. https://archive-ouverte.unige.ch/unige:11407