UNIGE document Scientific Article
previous document  unige:11407  next document
add to browser collection
Title

Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion

Authors
Brown, James
Gilbert, R. J.
Jones, E. Y.
Kiefer, Friedemann
Ruga, Pilar
show hidden authors show all authors [1 - 10]
Published in Molecular Biology of the Cell. 2005, vol. 16, no. 10, p. 4992-5003
Abstract The junctional adhesion molecules (JAMs) have been recently described as interendothelial junctional molecules and as integrin ligands. Here we show that JAM-B and JAM-C undergo heterophilic interaction in cell-cell contacts and that JAM-C is recruited and stabilized in junctional complexes by JAM-B. In addition, soluble JAM-B dissociates soluble JAM-C homodimers to form JAM-B/JAM-C heterodimers. This suggests that the affinity of JAM-C monomers to form dimers is higher for JAM-B than for JAM-C. Using antibodies against JAM-C, the formation of JAM-B/JAM-C heterodimers can be abolished. This liberates JAM-C from its vascular binding partner JAM-B and makes it available on the apical side of vessels for interaction with its leukocyte counter-receptor alpha(M)beta2 integrin. We demonstrate that the modulation of JAM-C localization in junctional complexes is a new regulatory mechanism for alpha(M)beta2-dependent adhesion of leukocytes.
Keywords AnimalsCell Adhesion/ physiologyCell Adhesion Molecules/genetics/ metabolismCell LineCoculture TechniquesCricetinaeCricetulusEndothelium/physiology/ultrastructureGreen Fluorescent Proteins/geneticsHumansImmunoglobulins/genetics/ metabolismIntercellular Junctions/ physiology/ultrastructureLeukocytes/ physiology/ultrastructureMacrophage-1 Antigen/ metabolismMiceMice, Inbred C57BLMicroscopy, ImmunoelectronPeptides/geneticsRecombinant Fusion Proteins/genetics/metabolism
Identifiers
PMID: 16093349
Full text
Structures
Citation
(ISO format)
LAMAGNA, Chrystelle et al. Dual interaction of JAM-C with JAM-B and alpha(M)beta2 integrin: function in junctional complexes and leukocyte adhesion. In: Molecular Biology of the Cell, 2005, vol. 16, n° 10, p. 4992-5003. https://archive-ouverte.unige.ch/unige:11407

182 hits

0 download

Update

Deposited on : 2010-08-27

Export document
Format :
Citation style :