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Presence of a very small population of Thy-1+, L3T4+ cells producing large amounts of IL-3 in young athymic nude mice

Kimoto, M.
de Kossodo, S.
Kindler, V.
Detraz, M.
Published in Immunology. 1989, vol. 68, no. 4, p. 557-563
Abstract A mixture of phorbol myristate acetate (PMA) and ionomycin was found to stimulate spleen and lymph node cells (LNC) from 6 to 8 week-old-athymic BALB/c nude mice, as well as from control +/+ mice, to secrete interleukin-3 (IL-3) in vitro. The specificity of the IL-3 bioassay was attested to by the use of rabbit anti-IL-3 antibodies, and by the detection of an accumulation of IL-3 mRNA. Cytotoxic treatment with relevant antibodies showed that the cells responsible for the IL-3 production in athymic nude mice was Thy-1+, L3T4+, Ly2-, while both L3T4+ and Ly 2+ cells produced IL-3 in control +/+ mice. Although the levels of IL-3 secreted by nude LNC varied among experiments, nude LNC were able to produce IL-3 at a level comparable to or higher than +/+ LNC. In addition, nude LNC consistently secreted two to three times more granulocyte-macrophage colony-stimulating factor (GM-CSF) than +/+ LNC, and the majority of GM-CSF secretion was dependent on the presence of L3T4+ cells. In contrast, IL-2 production by nude LNC was markedly limited. Since the flow microfluorometry analysis failed to demonstrate the presence of L3T4+ cells (less than 1%) in nude LNC, compared with 40-50% L3T4+ cells in +/+ LNC, our results suggest that athymic nude mice have a small population of Thy-1+, L3T4+ cells characterized by its ability to secrete IL-3 and GM-CSF at a very high rate.
Keywords AnimalsAntigens, Differentiation, T-Lymphocyte/ analysisAntigens, Surface/ analysisAntigens, Thy-1Cells, CulturedInterleukin-3/ metabolismIonomycinLymphocyte ActivationLymphocytes/immunology/ metabolismMiceMice, Inbred BALB CMice, NudeTetradecanoylphorbol Acetate
PMID: 2575077
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KIMOTO, M. et al. Presence of a very small population of Thy-1+, L3T4+ cells producing large amounts of IL-3 in young athymic nude mice. In: Immunology, 1989, vol. 68, n° 4, p. 557-563. https://archive-ouverte.unige.ch/unige:11379

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Deposited on : 2010-08-27

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