en
Scientific article
English

Recombinant murine IL-3 fails to stimulate T or B lymphopoiesis in vivo, but enhances immune responses to T cell-dependent antigens

Published inThe Journal of immunology, vol. 140, no. 6, p. 1889-1894
Publication date1988
Abstract

We have explored the in vivo effect of IL-3 on the lymphopoiesis and humoral responses of mice bearing osmotic minipumps loaded with murine rIL-3 for 1 to 4 wk. A marked splenomegaly due to the accumulation of hemopoietic precursors was seen, but no increase was found in the lymphoid organs in the total number of cells belonging to the T or B lymphocyte lineage, i.e., of L3T4+ or Lyt-2+, or of allospecific cytotoxic T lymphocyte precursor for the T lineage, or of sIg+ or B220+ cells, or of B colony-forming cells for the B lineage; total activity of natural killer and lymphokine-activated killer cells was decreased. In contrast to the splenomegaly, a marked diminution in the number of thymocytes was observed, suggesting that rIL-3 in large amounts does suppress the T lymphopoiesis, perhaps as the result of the selective stimulation of early progenitor cells toward the hemopoietic pathway. rIL-3 perfusion during immunization increased the IgM and IgG responses to a T cell-dependent antigen, human IgG, and prevented tolerance induction by the deaggregated human IgG, although in the same conditions it did not modify the response to a T cell-independent antigen. Our results suggest that in vivo IL-3 does not act directly on lymphocytes or their precursors, but may potentiate the humoral immune response to T cell-dependent antigens, presumably by acting on accessory cells.

Keywords
  • Animals
  • Antigens/ immunology
  • B-Lymphocytes/drug effects
  • Cell Differentiation/drug effects
  • Cell Division/drug effects
  • Female
  • Hematopoiesis/ drug effects
  • Interleukin-3/ pharmacology
  • Lymphoid Tissue/drug effects
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Recombinant Proteins/pharmacology
  • Splenomegaly
  • T-Lymphocytes/drug effects
Citation (ISO format)
KIMOTO, M. et al. Recombinant murine IL-3 fails to stimulate T or B lymphopoiesis in vivo, but enhances immune responses to T cell-dependent antigens. In: The Journal of immunology, 1988, vol. 140, n° 6, p. 1889–1894.
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ISSN of the journal0022-1767
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