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Title

Recombinant murine IL-3 fails to stimulate T or B lymphopoiesis in vivo, but enhances immune responses to T cell-dependent antigens

Authors
Kimoto, M.
Kindler, V.
Higaki, M.
Published in Journal of Immunology. 1988, vol. 140, no. 6, p. 1889-1894
Abstract We have explored the in vivo effect of IL-3 on the lymphopoiesis and humoral responses of mice bearing osmotic minipumps loaded with murine rIL-3 for 1 to 4 wk. A marked splenomegaly due to the accumulation of hemopoietic precursors was seen, but no increase was found in the lymphoid organs in the total number of cells belonging to the T or B lymphocyte lineage, i.e., of L3T4+ or Lyt-2+, or of allospecific cytotoxic T lymphocyte precursor for the T lineage, or of sIg+ or B220+ cells, or of B colony-forming cells for the B lineage; total activity of natural killer and lymphokine-activated killer cells was decreased. In contrast to the splenomegaly, a marked diminution in the number of thymocytes was observed, suggesting that rIL-3 in large amounts does suppress the T lymphopoiesis, perhaps as the result of the selective stimulation of early progenitor cells toward the hemopoietic pathway. rIL-3 perfusion during immunization increased the IgM and IgG responses to a T cell-dependent antigen, human IgG, and prevented tolerance induction by the deaggregated human IgG, although in the same conditions it did not modify the response to a T cell-independent antigen. Our results suggest that in vivo IL-3 does not act directly on lymphocytes or their precursors, but may potentiate the humoral immune response to T cell-dependent antigens, presumably by acting on accessory cells.
Keywords AnimalsAntigens/ immunologyB-Lymphocytes/drug effectsCell Differentiation/drug effectsCell Division/drug effectsFemaleHematopoiesis/ drug effectsInterleukin-3/ pharmacologyLymphoid Tissue/drug effectsMiceMice, Inbred BALB CMice, Inbred C3HMice, Inbred C57BLRecombinant Proteins/pharmacologySplenomegalyT-Lymphocytes/drug effects
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PMID: 3257991
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Other version: http://www.jimmunol.org/cgi/reprint/140/6/1889.pdf
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KIMOTO, M. et al. Recombinant murine IL-3 fails to stimulate T or B lymphopoiesis in vivo, but enhances immune responses to T cell-dependent antigens. In: Journal of Immunology, 1988, vol. 140, n° 6, p. 1889-1894. https://archive-ouverte.unige.ch/unige:11378

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Deposited on : 2010-08-27

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