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Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires

Publié dansThe Journal of immunology, vol. 165, no. 12, p. 6716-6722
Date de publication2000
Résumé

Intraepithelial lymphocytes (IEL) of the small intestine are anatomically positioned to be in the first line of cellular defense against enteric pathogens. Therefore, determining the origin of these cells has important implications for the mechanisms of T cell maturation and repertoire selection. Recent evidence suggests that murine CD8 alpha alpha intestinal IELs (iIELs) can mature and undergo selection in the absence of a thymus. We analyzed IEL origin by cell transfer, using two congenic chicken strains. Embryonic day 14 and adult thymocytes did not contain any detectable CD8 alpha alpha T cells. However, when TCR(+) thymocytes were injected into congenic animals, they migrated to the gut and developed into CD8alphaalpha iIELs, while TCR(-) T cell progenitors did not. The TCR V beta 1 repertoire of CD8 alpha alpha(+) TCR V beta 1(+) iIELs contained only part of the TCR V beta 1 repertoire of total iIELs, and it exhibited no new members compared with CD8(+) T cells in the thymus. This indicated that these T cells emigrated from the thymus at an early stage in their developmental process. In conclusion, we show that while CD8 alpha alpha iIELs originate in the thymus, T cells acquire the expression of CD8 alpha alpha homodimers in the gut microenvironment.

Mots-clés
  • Animals
  • Antigens, CD8/ biosynthesis
  • Base Sequence
  • Cell Aging/genetics/immunology
  • Cell Differentiation/genetics/immunology
  • Cell Movement/genetics/immunology
  • Chick Embryo
  • Chickens
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Intestinal Mucosa/cytology/ immunology/ metabolism
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell, alpha-beta/ biosynthesis/genetics
  • Receptors, Antigen, T-Cell, gamma-delta/biosynthesis
  • T-Lymphocyte Subsets/cytology/ immunology/ metabolism
  • Thymus Gland/ cytology/immunology/metabolism
Structure d'affiliation Pas une publication de l'UNIGE
Citation (format ISO)
IMHOF, Beat et al. Intestinal CD8 alpha alpha and CD8 alpha beta intraepithelial lymphocytes are thymus derived and exhibit subtle differences in TCR beta repertoires. In: The Journal of immunology, 2000, vol. 165, n° 12, p. 6716–6722.
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Article
accessLevelRestricted
Identifiants
ISSN du journal0022-1767
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Création27.08.2010 13:34:53
Première validation27.08.2010 13:34:53
Heure de mise à jour14.03.2023 16:03:42
Changement de statut14.03.2023 16:03:42
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