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Proceedings chapter
English

Rhizobium species NGR234 host-specificity of nodulation locus III contains nod- and fix-genes

Presented at Knoxville, July 14-19, 1996
PublisherSt. Paul : International Society for Molecular Plant-Microbe Interactions
Publication date1996
Abstract

Most nodulatlon genes of the broad host-range Rhizobium sp. NGR234 are located on the 536 kb symbiotic plasmid. Complete sequencing of the replicon revealed over 400 open-reading frames (ORF), many of which correspond to known genes. Combined physical/genetic analysis of the host specificity of nodulation (hsn) locus lll revealed many nodulation genes, some of which are essential for Nod-factor production (nodABQ and others which modify the Nod-factor structure in specific ways (noeE). When mutated, two other genes, ORF3 and fixF gave Fix⁻ phenotypes on Vigna unguiculata. fixF has a nuclear localisation sequence (NLS) which is functional in nuclear targeting in tobacco protoplasts. Mutations in fixF abolish production of a novel, rhamnose-rich lipopolysaccharide that is produced under conditions of flavonoid induction.

Citation (ISO format)
JABBOURI, Said et al. <i>Rhizobium</i> species NGR234 host-specificity of nodulation locus III contains <i>nod-</i> and <i>fix-</i>genes. In: Biology of plant-microbe interactions: proceedings of the 8th International Symposium on Molecular Plant-Microbe Interactions. Knoxville. St. Paul : International Society for Molecular Plant-Microbe Interactions, 1996. p. 319–324.
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Proceedings chapter (Published version)
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  • PID : unige:113185
ISBN0965462501
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