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Scientific article
English

The large subunit of HIV-1 reverse transcriptase interacts with beta-actin

Published inNucleic acids research, vol. 23, no. 5, p. 736-741
Publication date1995
Abstract

HIV-1 reverse transcriptase is a dimeric enzyme mainly involved in the replication of the viral genome. A filamentous phage cDNA expression library from human lymphocytes was used to select cellular proteins interacting with HIV-1 reverse transcriptase Affinity selections using the bacterially expressed monomeric large subunit of reverse transcriptase (p66) yielded host beta-actin. This clone was expressed as glutathione-S-transferase fusion protein which was identified by using a specific antibody against beta-actin. Furthermore we show that also the eukaryotic beta-actin binds to either the large subunit of reverse transcriptase or to the Pol precursor polyprotein in vitro. The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions.

Keywords
  • Actins/ metabolism
  • Animals
  • Base Sequence
  • Cattle
  • DNA Primers
  • DNA, Complementary
  • Gene Products, gag/metabolism
  • Glutathione Transferase/metabolism
  • HIV Reverse Transcriptase
  • HIV-1/ enzymology
  • Humans
  • Molecular Sequence Data
  • Protein Binding
  • Protein Precursors/metabolism
  • Protein Processing, Post-Translational
  • RNA-Directed DNA Polymerase/ metabolism
  • Recombinant Fusion Proteins/metabolism
  • gag Gene Products, Human Immunodeficiency Virus
  • pol Gene Products, Human Immunodeficiency Virus
Affiliation Not a UNIGE publication
Citation (ISO format)
HOTTIGER, M. et al. The large subunit of HIV-1 reverse transcriptase interacts with beta-actin. In: Nucleic acids research, 1995, vol. 23, n° 5, p. 736–741.
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ISSN of the journal0305-1048
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